Radiation does not alter AKT and MTOR gene expression.

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Radiation does not alter AKT and MTOR gene expression. Radiation does not alter AKT and MTOR gene expression. A, Schematic of the AKT/mTOR signaling network, which was included in the gene array. B, mRNA expression of indicated genes in 3D-cultured PC3 cells and PC3 xenografts irradiated with a SD of 10 Gy or with a multifractionated regimen of five times 2 Gy or 10 times 1 Gy (2 Gy per day). Cells were lysed at 24 hours after the final radiation dose. After RNA isolation, cDNA was synthesized, and qRT-PCR was performed. mRNA expression was determined using the RT² Profiler PCR Array (Human PI3K-AKT Signaling Pathway) from Qiagen. Results show mean ± standard deviation (STDEV) (n = 3). Iris Eke et al. Mol Cancer Ther 2018;17:355-367 ©2018 by American Association for Cancer Research