Cells lacking CDK6 kinase function are required to mutate p53.

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Cells lacking CDK6 kinase function are required to mutate p53. Cells lacking CDK6 kinase function are required to mutate p53. A, cDNA of the protein-coding p53 transcript was amplified by PCR and analyzed by Sanger sequencing. The substituted amino acids as well as the corresponding human mutations are indicated. B, Annexin V/7-AAD staining of Cdk6−/− cells treated with etoposide (1 μmol/L), PRIMA-met (10 μmol/L), or the combination of both drugs for 24 hours. Numbers represent the mean ± SD (n = 3 cell lines per genotype; one representative example is depicted). C, Cell-cycle profiles analyzed by propidium iodide (PI) staining of BCR–ABL-transduced bone marrow cells at day 19. The gating strategy is indicated in the histogram plots; bar graphs summarize our experiments (n = 3 different biological replicates). D,Trp53+/+;Cdk6+/+, Trp53+/+;Cdk6−/−, Trp53−/−;Cdk6+/+ and Trp53−/−;Cdk6−/− bone marrow cells were transduced with BCR–ABL. The panels show representative Annexin V/7-AAD stainings at day 19. The numbers represent the mean ± SD (n = 3 different biological replicates). Florian Bellutti et al. Cancer Discov 2018;8:884-897 ©2018 by American Association for Cancer Research