Low pH represses growth of cells with the hyphal morphology and promotes growth as pseudohyphae. Low pH represses growth of cells with the hyphal morphology.

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Low pH represses growth of cells with the hyphal morphology and promotes growth as pseudohyphae. Low pH represses growth of cells with the hyphal morphology and promotes growth as pseudohyphae. (A) GlcNAc promotes hyphal induction in citrate-buffered medium. Representative images show cells grown in the indicated medium for 3 h at 37°C in YNB or YNBN medium buffered to pH 7 with phosphate or citrate, and counts are from 3 independent experiments. (B and C) Medium buffered to pH 4 promoted pseudohyphal growth. Representative images of cells grown for 3 h in YNBNC at pH 4 and 7 are shown (B), and quantitative enumeration of cellular morphologies is summarized (C). Error bars represent one standard deviation. Jeffrey M. Hollomon et al. mSphere 2016; doi:10.1128/mSphere.00283-16