Integration of the kDNA minicircle sequence into the genome of a rabbit with Chagas' disease. Integration of the kDNA minicircle sequence into the genome.

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Integration of the kDNA minicircle sequence into the genome of a rabbit with Chagas' disease. Integration of the kDNA minicircle sequence into the genome of a rabbit with Chagas' disease. (A) Hybridization of rabbit DNA with a specific kDNA probe. A total of 20 μg of EcoRI-digested DNA separated on a 0.7% agarose gel was used for Southern hybridization with 1 μg of a T. cruzi cloned kDNA constant region (kCR) probe. (B) Schematic representation of kDNA integration into rabbit DNA. Arrows show primers used for 5′ RACE to detect the kDNA insertion into the rabbit genomic clone LBNL1. The integration of CCA/ACC-rich kDNA (bp 1255 to 1907) occurred within rabbit DNA, showing attachment sites of direct short CACCAACC repeats. An ORF spans the chimeric sequence at bp 1217 to 1582. (Reprinted from reference 293 with permission of the publisher [see comments regarding this retracted article at first citation].)‏ Antonio R. L. Teixeira et al. Clin. Microbiol. Rev. 2011; doi:10.1128/CMR.00063-10