PTB-independent ShcA pools employ distinct domains to hyperactivate mTOR and Src signaling. PTB-independent ShcA pools employ distinct domains to hyperactivate.

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PTB-independent ShcA pools employ distinct domains to hyperactivate mTOR and Src signaling. PTB-independent ShcA pools employ distinct domains to hyperactivate mTOR and Src signaling. A, Immunoblot analysis with the indicated antibodies in ErbB2-driven breast cancer cells expressing specified FLAG-tagged ShcA alleles. The data are representative of three independent experiments. *, P < 0.05. B and C, IHC staining of tumors using pS240/244-rS6 and pY416-Src–specific antibodies, respectively. The data depict the average positively stained cells or pixels ± SEM is representative of 6–8 tumors per group. *, P < 0.05; ***, P < 0.001. D, FLAG immunoprecipitates were probed with pY239/240-ShcA or ShcA-specific antibodies by immunoblot analysis. Both DMSO and PP2 (2 μmol/L)-treated cells were assayed. The barplot represents densitometric quantification of three independent experiments using ImageJ software. One-way ANOVA (DMSO or PP2)—ShcAWT versus PTBMUT: **, P < 0.01; ****, P < 0.0001; PTBMUT vs. PTBMUT/3F: δδ, P < 0.01. We also compared the differences in ShcA tyrosine phosphorylation in PTBMUT (DMSO vs. PP2) and PTB/SH2MUT (DMSO vs. PP2) using a two-tailed, paired t test (#, P < 0.05). E, FLAG immunoprecipitates from indicated cell lines were probed with pY416-Src, Src, Fyn, Lyn, and FLAG-specific antibodies via immunoblot analysis. The barplot represents densitometric quantification of three independent experiments using ImageJ software. PTBMUT versus PTB/SH2MUT: δ, P < 0.05. F, Clonogenic assays of specified cell lines treated with DMSO and lapatinib (0.5 μmol/L). The data is depicted as a fold change in viability relative to DMSO control and is representative of three independent experiments (means ± SEM). ShcAWT versus PTBMUT: ***, P < 0.001; PTBMUT vs. PTBMUT/3F or PTB/SH2MUT: δ, P < 0.05; δδ, P < 0.01. Jacqueline R. Ha et al. Mol Cancer Res 2018;16:894-908 ©2018 by American Association for Cancer Research