A user's perspective on GeoMxTM digital spatial profiling

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A user's perspective on GeoMxTM digital spatial profiling Trieu My Van, Christian U. Blank Immuno-Oncology Technology Volume 1, Pages 11-18 (July 2019) DOI: 10.1016/j.iotech.2019.05.001 Copyright © 2019 Terms and Conditions

Figure 6 Correlation of digital spatial profiling (DSP) counts with immunofluorescent or standard immunohistochemical staining. (A) Tonsil tissue stained with PanCK (green), CD45 (red) and CD3 (yellow) visualization markers. Scale bar 100 μm. Graphs depict nuclei and housekeeping (HK)-normalized DSP counts of indicated proteins in ‘tumor’ or ‘immune’-enriched regions of interest (ROIs). (B) Nuclei and HK-normalized counts for CD163 and PD-L1 obtained from melanoma patient groups 1 (n=22) and 2 (n=32). Each dot represents one ROI/patient selected by similar tissue morphology. *P<0.005, unpaired t-test. ns, non-significant. (C) Representative images for CD163 and PD-L1 were obtained from melanoma tissue samples from groups 1 and 2 using standard immunohistochemistry. Scale bar 200 μm. Immuno-Oncology Technology 2019 1, 11-18DOI: (10.1016/j.iotech.2019.05.001) Copyright © 2019 Terms and Conditions