Increased migration and podia formation in NFIB-suppressed human osteosarcoma cells. Increased migration and podia formation in NFIB-suppressed human osteosarcoma.

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Increased migration and podia formation in NFIB-suppressed human osteosarcoma cells. Increased migration and podia formation in NFIB-suppressed human osteosarcoma cells. Subconfluent osteosarcoma cells were infected with the indicated siRNAs, and 48 hours after infection, wounds were made on the monolayers. Bright field images were taken at time 0 and 16, 24, and/or 30 hours (h). The wound-healing cell migration and filamentous actin staining assays were done in triplicate for three cell lines: OSA (A), HOS (B), and U2OS (C). The rs7034162 genotype is shown for the osteosarcoma cell lines: U2OS and HOS cells carry the homozygous non–risk allele (TT), and OSA cells carry the homozygous risk allele (AA). A representative example of the three independent experiments performed is shown. The cells showed increased migration after treatment with siRNA against NFIB (siNFIB) compared with the siNEG control, and the filamentous actin staining (far right) shows increased podia formation in the cells treated with siNFIB. D, soft-agar colony-formation assay in OSA, HOS, and U2OS cell lines. The HOS and OSA cells showed a significant reduction in colony formation after overexpression of NFIB compared with the Luciferase (Luc) control. Lisa Mirabello et al. Cancer Discovery 2015;5:920-931 ©2015 by American Association for Cancer Research