Pre-GMP Facility at ANA FUTURA Preliminary advanced equipment A new facility for safe translation of ATMPs from the lab to the clinic What can you do at the Pre-GMP facility? HQ plasmid production and purification Viral vector production and purification Cell expansion and formulation in automated, closed systems Gene transfer by flow electroporation in closed system Isolation and purification of extracellular vehicles What would you like to do? Purposes of the Pre-GMP facility Make research projects ready for transition to GMP production Develop and validate SOPs Test new equipment Education and staff training At the ANA FUTURA building at KI Campus Flemingsberg 10.000 m2 of newly rebuilt laboratory and office space with >300 world leading scientists, clinicians, and teachers Access to advanced facilities for: Bio Safety Level-3 laboratory for developing new viral vector systems Complete full service FACS facility for cells sorting and analysis Mass spectrometry for proteomics and metabolomics Confocal microscopy 5 fully equipped rooms for: cell expansion, vector and plasmid production and purification, multiple projects development Office space and archives Design 500 m2 5 clean rooms 3 locked offices Storage and – 80oC freezers In each clean room Class II LAF benches CO2 incubators Table top and high capacity centrifuges Bright field microscopes 2 Fridge/Freezer ANA FUTURA building Campus Flemingsberg Pre- GMP Facility Preliminary advanced equipment HQ plasmid production Fermentors TFF/ÄKTA Viral vector production Transduction/electroporation Virus/particle quantificantion TFF/ÄKTA (purification) Quantum (packaging cell culture) Cell culture and expansion Xuri Quantum Milteniy Prodigy Location: Karolinska Institutet Campus Flemingsberg ANA Futura Floor 7 Alfred Nobels Alle 8 141 52 Huddinge Contact Information: Anna Pasetto, PhD Managing Director Pre-GMP facility 08-52483857; 0702689758 anna.pasetto@ki.se