Scanning Electron Microscopy

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Scanning Electron Microscopy (SEM)

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Presentation transcript:

Scanning Electron Microscopy Scanning Electron Microscope Compositional Imaging (Backscatter Electron, BSE) FEI Quanta 650 FEG (SEM) The SEM in the laboratory features a high-resolution field emission gun with environmental technology. It is one of the most versatile SEM instruments available. In the lab we look at everything from cancer cells to car parts. In BSE mode, regions with elements of higher atomic number show higher intensity in the image Image: Ceramic Wet STEM High Vacuum Wet scanning-transmission electron microscopy (STEM) allows high-resolution transmission imaging of biological samples in a hydrated state, with minimal sample preparation. Image: PLGA Micro-balloons The traditional SEM method. In high vacuum mode it is possible to obtain surface images of dry, conductive samples with nanometer resolution Image: Porous Manganese Carbonate Templates (Cover of JMC_B) Courtesy of Dr. Kharlampieva Low Vacuum Low vacuum is a variable pressure mode, allowing for imaging of non-conductive, dry samples. Image: Mouse Intestine Image Processing Techniques Consult with Nik for your image processing needs. Examples of image processing work done: Porosity metrics Cell/Grain Size Dendrite Arm Spacing Graphite Spacing Application specific image montages Various post processing algorithms for HRIF Colorization Environmental SEM (ESEM) ESEM is a mode which can be used to image wet, living, and unfixed samples Image: Sea Urchin Energy Dispersive Spectroscopy (EDS) EDS provides a qualitative elemental analysis of samples. This is available for all pressure modes Image: Pyrite Other Beam Deceleration Stereo Imaging Topography And more! Image: Diamond on a steel substrate HRIF Sponsors UAB Institutional Cores Program Comprehensive Cancer Center (P30 CA013148) Rheumatic Disease Core Center (P30 AR048311) Veteran’s Administrative Hospital Research Division Comprehensive Arthritis Musculoskeletal Bone and Immunity Center Additional SEM Lab Sponsors: Department of Materials Science & Engineering College of Arts and Sciences Center for Nanoscale Materials and Biointegration Nik Jindal, SEM Engineer (205) 934-8427 NJindal@uab.edu Alexa Mattheyses, PhD, Director (205) 975-0680 mattheyses@uab.edu