Growth curve (a) and doubling time (b) of glioma cells.

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Growth curve (a) and doubling time (b) of glioma cells. Growth curve (a) and doubling time (b) of glioma cells. For every cell line, a total of 18 wells on three six-well plates were seeded with 30,000 cells/well on day 1. Cells from three of the 18 wells were counted daily starting on day 2 for a total of 6 days. Doubling time of each cell line was calculated by plotting logarithmic average cell numbers against their day of harvesting. Two points corresponding to 100,000 cells (log 5) and 200,000 cells (log 5.3) were located on each of the curves. Subsequently, two other points on the horizontal axis (day of harvesting) were located by drawing vertical lines from the two points on the curve toward the horizontal axis. Using distance on the axis of day of harvesting, the doubling time was calculated. Note that A-172 and U-87 MG cells had similar doubling times, as did LG-11 and NG-1 cells. Li Dao Ke et al. Clin Cancer Res 2000;6:2562-2572 ©2000 by American Association for Cancer Research