The effect of different concentrations of WMC-79 and time of exposure on the growth of HCT-116 cells. The effect of different concentrations of WMC-79 and time of exposure on the growth of HCT-116 cells. A, cells grown in 96-well plates were exposed to various concentrations of the drug for different times and then incubated up to 120 h. The effect of the treatment was determined using MTT cell proliferation assay. Points, mean of three independent experiments; bars, SD. B, viable cell number counting. Cells (1 × 106) were plated in 75-cm2 flasks and, after attachment (24 h later), were exposed to 0, 10, and 100 nmol/L WMC-79. Following trypsin-mediated detachment, cells were counted at the indicated times by hemocytometry using trypan blue exclusion for cell viability. Each number represents the average of triplicate experiments. Teresa Kosakowska-Cholody et al. Mol Cancer Ther 2005;4:1617-1627 ©2005 by American Association for Cancer Research