Effect of hydrogen peroxide on survival and transcriptional activation of TFF1 in established cell lines from the following human tumor tissues: breast.

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Effect of hydrogen peroxide on survival and transcriptional activation of TFF1 in established cell lines from the following human tumor tissues: breast (MCF7), stomach (HS746T), and colon (LS180). Effect of hydrogen peroxide on survival and transcriptional activation of TFF1 in established cell lines from the following human tumor tissues: breast (MCF7), stomach (HS746T), and colon (LS180). A, clonogenic survival. B, time course of TFF1 mRNA induction by H2O2 (0.5 mm for 1 h) under standard estrogenic medium conditions, i.e., cells were cultured in medium containing unmodified serum and phenol red. Values are expressed relative to mRNA level in contemporaneous controls. Coinduction of TFF1 and TFF3 mRNA was detected only in LS180 cells (see Table 1). Bars, SE. C, example of Northern analysis of TFF1 mRNA in total RNA samples (10–20 μg/lane) isolated from HS746 cells. A representative sample of ethidium bromide-stained membrane is shown to demonstrate RNA quality and equal loading. Elizabeth K. Balcer-Kubiczek et al. Mol Cancer Ther 2002;1:405-415 ©2002 by American Association for Cancer Research