Allegation.

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Presentation transcript:

Allegation

Raw Scanned data File generated after experiments from Gel Doc system Top Gel Top Gel Bottom Gel Bottom Gel Gel 3 Top Gel

Labeled Scanned data File generated after experiments from Gel Doc system Top Gel Marker Top Gel 1M(BA) 2N(BA) 5M(MA) 6N(MA) 3N(MA) 9M(BA) 10N(BA) 33M(BA) 34N(BA) 11M(BA) 12N(BA) 13M(BA) 14N(BA) 23M(MA) 43M(BA) 44N(BA) 25M(BA) 26N(BA) Marker Marker 15M(BA) 16N(BA) 17M(BA) 18N(BA) 21M(BA) 22N(BA) 19M(MA) 25M(BA) 26N(BA) 39M(BA) 40N(BA) 41M(MA) 42N(MA) 45M(BA) 31M(MA) 47M(BA) 48N(BA) 31N(BA) Marker Marker Bottom Gel Bottom Gel Gel 3 Top Gel 35M(BA) 36N(BA) 37M(BA) 27M(MA) 39M(BA) 40N(BA) 46N(BA) 29M(MAS) 32N(BA) Marker

Error while cropping and naming Error while cropping and naming Samples are named from the above respective Gel (1,2, and 3) Top and Bottom gels * * * * * * 10N(BA) Gel 2 Top Gel Marker 1M(BA) 2N(BA) 5N(MA) 6M(MA) 3N(MA) 9M(BA) Marker 11M(BA) 14N(BA) 23M(MA) 12N(BA) 13M(BA) 43M(BA) 44N(BA) 300bp 292bp 300bp 292bp 227bp 227bp Gel 1 Top Gel 65bp 65bp * * * * * $ Marker 15M(BA) 16N(BA) 17M(BA) 18N(BA) 21M(BA) 22N(BA) 19M(MA) Marker 35M(BA) 36N(BA) 37M(BA) 27M(MA) 39M(BA) 40N(BA) 46N(BA) 29M(MAS) 300bp 292bp 292bp 227bp 300bp 227bp Gel 3 Top Gel Gel 1 Bottom Gel 65bp 65bp * * * Error while cropping and naming the gel $ Error while cropping and naming the gel Marker 39M(BA) 40N(BA) 41M(MA) 42N(MA) 45M(BA) 31M(MA) 47M(BA) 48N(BA) 300bp 292bp 227bp Gel 2 Bottom Gel 292bp Allegation Answer’s: 65bp 227bp Since we have cropped all the double bands for biallelic samples and generating the respective images for Journal publication and placing them in the final image error occurred while generating the final image for sample (17,18) (21,22) (39,40) and above is the exact original gels what we have used and all the respective double band sample are biallelic, what we should have used for publication and discussion and this does not change the data anyways. The erratum can be raised to the journal and get it updated in the published journal if needed. 65bp

Allegation Figure2, Same highlighted bands marked in the below cropped gels are used duplicated for sample (11, 12) to (21 ,22) and triplicated for samples (15,16) (17,18) and (39,40) in the Figure 2 * * * * * * * Marker 1M(BA) 2N(BA) 5M(BA) 6N(BA) 9M(BA) 10N(BA) 11M(BA) 12N(BA) 13M(BA) 14N(BA) 15M(BA) 16N(BA) 17M(BA) 18N(BA) 300bp 292bp 227bp 50bp 65bp Right Cropped Right Cropped Wrong Cropped * * * * * * Marker 21M(BA) 22N(BA) 35M(BA) 36N(BA) 39M(BA) 40N(BA) 43M(BA) 44N(BA) 45M(BA) 46N(BA) 47M(BA) 48N(BA) 292bp 300bp 227bp 50bp 65bp Wrong Cropped Wrong Cropped