Analysis of mRNA and protein levels of Loricrin, COMP, CXCL9, KRT19, and CYP 3A5 genes in OSFs compared with normal controls. Analysis of mRNA and protein levels of Loricrin, COMP, CXCL9, KRT19, and CYP 3A5 genes in OSFs compared with normal controls. A. RT-PCR analysis of five genes of representative five couples of samples [5 OSF (O) and 5 NBM samples (N)]. M, DNA marker. B. mRNA ratios of Loricrin, COMP, CXCL9, KRT19, and CYP 3A5 in OSF versus normal controls. The gene expression level was normalized by GAPDH as internal control. C. Western blotting analysis of five proteins of representative three couples of samples (3 OSF and 3 NBM). D. Protein ratios of COMP, CXCL9, KRT19, and CYP 3A5 in OSF versus normal controls. The protein expression level was normalized by using β-actin as internal control. Ning Li et al. Cancer Epidemiol Biomarkers Prev 2008;17:2249-2259 ©2008 by American Association for Cancer Research