Combined inhibition of Wee1 and PARP causes persistent radiation-induced DNA damage. Combined inhibition of Wee1 and PARP causes persistent radiation-induced.

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Combined inhibition of Wee1 and PARP causes persistent radiation-induced DNA damage. Combined inhibition of Wee1 and PARP causes persistent radiation-induced DNA damage. AsPC-1 (A, B) and MiaPaCa-2 (C, D) cells were treated with AZD1775/olaparib for 1 hour pre- and for the indicated times post-radiation (RT) or mock-radiation. At each time point post-radiation, cells were fixed for flow cytometric analysis and analyzed for γH2AX positivity and DNA content. Data shown are the mean percentage of γH2AX-positive cells ± SE from n = 3 to 4 independent experiments. Statistical significance (P < 0.05) is indicated versus radiation*, AZD1775 + radiation‡, and olaparib + radiation¥. David Karnak et al. Clin Cancer Res 2014;20:5085-5096 ©2014 by American Association for Cancer Research