Induction of DNA strand breaks by artesunate.

Slides:

Advertisements

Similar presentations

Δ-Tocotrienol treatment is more effective against hypoxic tumor cells than normoxic cells: potential implications for cancer therapy Akira Shibata, Kiyotaka.

Advertisements

From: IGF-1 Regulates the Extracellular Level of Active MMP-2 and Promotes Müller Glial Cell Motility Invest. Ophthalmol. Vis. Sci ;56(11):

Enhanced DNA damage in IDH1 mutant glioma cells.

DNA damage foci in irradiated cells.

Camptothecin‐induced DNA damage response is ataxia telangiectasia mutated dependent in post‐mitotic cells. Camptothecin‐induced DNA damage response is.

Induction of DNA double‐strand breaks and activation of the ataxia telangiectasia mutated pathway in post‐mitotic cells in response to camptothecin. Induction.

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

Binding of 53BP1 on uncapped telomeres.

DNA-damage (γ-H2AX) and telomere damage–induced foci (TIFs).

PDGF regulates gap junction communication and connexin43 phosphorylation by PI 3- kinase in mesangial cells Jian Yao, Tetsuo Morioka, Takashi Oite Kidney.

NM-3 induces p21 levels and down-regulation of Cdk2 activity.

Tatsushi Toyooka, Mio Ishihama, Yuko Ibuki

STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs.

Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active.

Fig. 2. BET inhibition enhances PARPi-induced DNA damage.

TDP1 knockdown increases the sensitivity of rhabdomyosarcoma cell lines to CPT treatment. TDP1 knockdown increases the sensitivity of rhabdomyosarcoma.

Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were.

eIF5A-PEAK1 signaling regulates KRAS protein expression.

Effects of SYCE2 expression on DSB repair.

High-affinity binding to the hypervariable region of the β1 I-like domain controls signaling to fibronectin fibrillogenesis. High-affinity binding to the.

CML-HSA treatment activates MAPK family members ERK1/2 and p38 but not JNK. Samples were taken at the indicated times after 100 μg/ml CML-HSA exposure.

Curcumin-generated ROS activates Chk1 and Chk2 kinases.

Induction of apoptosis by NS398.

CDCP1 is required for invadopodia formation and ECM degradation by human breast cancer cells. CDCP1 is required for invadopodia formation and ECM degradation.

Protracted temozolomide (TMZ) treatment leads to acquired TMZ resistance in glioblastoma (GBM) cells in vivo. Protracted temozolomide (TMZ) treatment leads.

BME treatment increases granzyme B expression in NK 3.3 cells.

DNA damage signaling regulates mutant p53 ubiquitination.

DNA recombination at sites of replication damage in BLM-deficient cells. DNA recombination at sites of replication damage in BLM-deficient cells. A. Nucleotide.

Distribution of γ-H2AX foci on metaphase spreads from BLM-proficient and BLM-deficient cells. Distribution of γ-H2AX foci on metaphase spreads from BLM-proficient.

Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells. Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells.

Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.

Geldanamycin-induced endocytosis and down-regulation of ErbB2 in cells expressing ErbB2 only. Geldanamycin-induced endocytosis and down-regulation of ErbB2.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Induction of cell death in prostate cancer cells by SAHA and ZOL

Effect of UV irradiation on PTHRP gene expression.

DNA damage-induced RPA foci in asynchronous cells labeled with BUdR.

LY induces cytotoxicity in hMECs via inhibition of Akt/p70S6K kinase activities. LY induces cytotoxicity in hMECs via inhibition of Akt/p70S6K.

Biological effects of BRAF silencing: growth curves of A375 (A) and ARO (B) cell lines in the absence or presence of doxycycline. Biological effects of.

N-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. n-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. HEC-1-A.

Alkaline Comet assay showing DNA break formation after 6-TG treatment, implying more SSBs generated in MMR+ cells. Alkaline Comet assay showing DNA break.

Tipifarnib combined with bortezomib induces cell death in diverse multiple myeloma and AML cell lines. Tipifarnib combined with bortezomib induces cell.

Dual apoptotic signaling pathways triggered by IMMU-140: Anti–HLA-DR- and SN-38–mediated signals. Dual apoptotic signaling pathways triggered by IMMU-140:

Contrasting results on enhanced DNA strand break repair in CD133+ cells. Contrasting results on enhanced DNA strand break repair in CD133+ cells. A. CD133+

Staurosporine induces changes in MMP

A, Northern blot analysis of DRCTNNB1A.

Ketogenic diets combined with fractionated radiation treatment results in decreased immunoreactive PCNA in tumor tissue. Ketogenic diets combined with.

Changes in signal transduction pathway induced by gefitinib.

Ataxia telangiectasia and DNA-dependent protein kinase (DNA-PK) catalytic subunit-deficient cell lines efficiently form H2AX foci after exposure to ionizing.

Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. Characterization of DNA-PK and PARP-1 levels and activities in the.

BEZ235 inhibits DNA-PK and enhances G2–M growth arrest after radiation

6-TG dose and time relationships for DSB formation using PFGE, demonstrating that the formation of DSBs in both MMR+ M4 and MMR− V2 cells is dose dependent.

Effect of CDV on human SF7796 xenografts in vivo.

Γ-H2AX foci formation in ATM−/− and DNA-dependent protein kinase catalytic subunit (DNA-PKcs)−/− mouse embryo fibroblasts (MEFs) but not in ATM−/− MEFs.

The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. Serum-starved.

EMT alters activation of AKT and serum-independent proliferation.

DNA-binding and double-strand break formation by Vpr.

Induction of apoptosis by statins in NB4 cells and NB4 variant cell lines. Induction of apoptosis by statins in NB4 cells and NB4 variant cell lines. A,

Purified Vpr induces double-strand breaks in vitro.

EGCG affects growth factor receptor signaling in H2111, H358, and H460 NSCLC cells. EGCG affects growth factor receptor signaling in H2111, H358, and H460.

Effect of bevacizumab on the proliferation of A2780 cells.

Sorafenib inhibits cell proliferation and induces apoptosis in HCC cells. Sorafenib inhibits cell proliferation and induces apoptosis in HCC cells. A,

RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis in ovarian cancer cells. RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis.

Influence of minocycline on TGF-β1 expression and TGF-β1–induced NF-кB activation in ovarian cancer cells. Influence of minocycline on TGF-β1 expression.

AR promotes DNA double-strand break resolution.

LY elicits hyperphosphorylation of Akt.

Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated breast cancer cells. Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated.

EGF induces HPSE nucleolar localization in human BMBC cells.

Tobacco-specific carcinogens induce methylation, DNA adducts, DNA damage, and activation of DNMT1. Tobacco-specific carcinogens induce methylation, DNA.

WEE1 inhibition followed by TRAIL treatment results in apoptotic cell death in MB231 cells. WEE1 inhibition followed by TRAIL treatment results in apoptotic.

Determination of Per2 (left), MTNR1A (middle), and ERβ (right) protein expression levels in mammary tissues of rats sacrificed at ZT12. Determination of.

Presentation transcript:

Induction of DNA strand breaks by artesunate. Induction of DNA strand breaks by artesunate. A, exponentially growing CHO-9 cells were exposed to artesunate for 24 h, harvested, and subjected to alkaline single-cell gel electrophoresis. Points, mean of three independent experiments; bars, SD. B, CHO-9 cells were exposed to artesunate (ART) for 24 h, harvested, and subjected to Western blot analysis. As loading control, the filter was reincubated with extracellular signal-regulated kinase 2 (Erk2). Induction factors (IF) were calculated by densitometric measurements of γ-H2AX and related to Erk2. C, immunohistochemical staining of cells not treated (Control) and treated with artesunate for 24 h. Nuclei were stained red with propidium iodide; γ-H2AX foci are the green fluorescent dots on the red background. D, quantification of γ-H2AX foci in CHO-9 cells treated for 24 h with artesunate. Paul C.H. Li et al. Cancer Res 2008;68:4347-4351 ©2008 by American Association for Cancer Research