EGFR signaling and ADAM10 are required for N-cadherin processing.

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EGFR signaling and ADAM10 are required for N-cadherin processing. EGFR signaling and ADAM10 are required for N-cadherin processing. A, EGFR signaling induces cell surface shedding of N-cadherin in SVZ NPC cultures. Following EGF simulation for the indicated time points, Sulfo-NHS-biotin was used to label cell surface proteins with exposed lysine residues (i.e., N-cadherin), and the labeled material (membrane fraction) was precipitated with streptavidin beads. Anti-N-cadherin was used with the lysate collected from the membrane fraction to determine the processing state of N-cadherin (unprocessed/full-length or processed). Following EGF stimulation, immunoreactivity against full-length N-cadherin markedly decreases, indicating increased processing in response to EGFR activity. Equal aliquots of intracellular lysate were analyzed by WB with anti-β-Actin, to demonstrate equal amounts of material were collected, and subsequently used as input for the above analysis. B, Diagram indicating the workflow for analysis of N-cadherin shedding used in (C). Equal numbers of SVZ NPCs were plated and maintained in vitro. Following EGF stimulation for the indicated time points, the culture media was collected for immunoprecipitation and Western blot (WB) analysis to detect the 95 kDa fragment of N-cadherin released upon shedding. C, NPCs from the SVZ of the wild-type, ADAM10 fl/fl, and Wa2/Wa2 mice were treated with EGF in vitro for 0, 4, or 24 h. Wild-type mice showed an accumulation of the 95 kDa fragment, the product of N-cadherin cleavage, in the supernatant. Note that accumulation of the 95 kDa fragment was not detected in NPCs isolated from ADAM10fl/fl or Wa2/Wa2 mice after EGF stimulation, indicating cleavage does not occur at detectable levels in response to treatment. D, Adult ADAM10fl/fl mice received three tamoxifen injections 24 h apart over the course of 3 d to ablate the expression of ADAM10 in nestin+ cells of the SVZ. Following tamoxifen treatment, SVZ tissue was collected and processed for WB analysis. N-cadherin shedding and downstream signaling components of the N-cadherin pathway (β-catenin and pp120) were reduced in ADAM10fl/fl mice compared with control samples; n = 3. Arrow, Full-length N-cadherin; line, CTF. Michael Klingener et al. J. Neurosci. 2014;34:9590-9606 ©2014 by Society for Neuroscience