Treatment with DZNep depletes expression of PRC2 proteins EZH2, SUZ12, and associated 3MeK27H3 levels in cultured human MCL cells. Treatment with DZNep.

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Treatment with DZNep depletes expression of PRC2 proteins EZH2, SUZ12, and associated 3MeK27H3 levels in cultured human MCL cells. Treatment with DZNep depletes expression of PRC2 proteins EZH2, SUZ12, and associated 3MeK27H3 levels in cultured human MCL cells. A, immunoblot analyses of EZH2, SUZ12, EED, BMI1, and β-actin in JeKo-1 cells following 24 hours treatment with DZNep. B, immunoblot analyses of EZH2, SUZ12, EED, BMI1, and β-actin in primary MCL cells after treatment with DZNep for 24 hours. C, quantitative RT-PCR for EZH2 and SUZ12 in MO2058 and JeKo1 treated with DZNep for 16 hours. The relative quantity (RQ) of each mRNA was normalized against glyceraldehyde-3-phosphate dehydrogenase expression. D, immunoblot analyses of 3Me K27H3, 3MeK4H3, 3MeK79H3, 3MeK9H3, AcK16H4, AcK27H3, AcK56H3, and histone H3 on acid-extracted histones from JeKo-1 cells treated with DZNep for 24 hours. E, JeKo-1 cells were treated with DZNep for 4 hours. Following this, EZH2 was immunoprecipitated and immunoblot analyses were conducted for DNMT1, SUZ12, EED, and EZH2 on the immunoprecipitates. The numbers beneath the blots represent densitometric evaluation of band intensity of the proteins bound to EZH2 in the immunoprecipitates. Warren Fiskus et al. Clin Cancer Res 2012;18:6227-6238 ©2012 by American Association for Cancer Research