BAF57 binds AR DBD/hinge region.

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BAF57 binds AR DBD/hinge region. BAF57 binds AR DBD/hinge region. A, immobilized GST or GST-BAF57 was incubated with [35S]Met-labeled AR mutants or CD44 (negative control) overnight at 4°C, washed, and subjected to SDS-PAGE. Five percent input and bound protein were detected by autoradiography. B, immobilized GST or GST-AR fusions were incubated with [35S]Met-labeled BAF57 and pull-down analysis was carried out as in A. Kevin A. Link et al. Cancer Res 2008;68:4551-4558 ©2008 by American Association for Cancer Research