Repair fidelity studies with increasing concentrations of antibodies against NHEJ repair proteins. Repair fidelity studies with increasing concentrations.

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Repair fidelity studies with increasing concentrations of antibodies against NHEJ repair proteins. Repair fidelity studies with increasing concentrations of antibodies against NHEJ repair proteins. A, C, and EPCR products of three to six randomly selected misrepaired colonies from LacZa reactivation experiments using HL60 nuclear extracts incubated with increasing concentrations of antibodies to NHEJ proteins: Anti-Ku86 (A; 1:100–1:20) anti-Ku70 (1:100–1:20) and Anti-DNA-PKcs (B). b, blue colony and L, DNA ladder (bp). B, D, and F graphs showing the percentage aberrant deletions with increasing antibody concentration. B, experiments using anti-Ku86. HL60 + anti-Ku86 ( ) and AML extract + anti-Ku86 ( , ····). D, experiments using anti-Ku70, HL60 + anti-Ku70 (▪), and AML extract + anti-Ku70 (▪, ····). F, experiments using anti-PKcs, HL60 + anti-DNA-PKcs (▴), and AML extract + anti-DNA-PKcs (▴, ····). White PCR products < 600 bp (>28 bp deletion) were considered aberrant, and thus, the percentage of aberrant deletions was derived by dividing the number of aberrant PCR products by the total number of colonies. Terry J. Gaymes et al. Cancer Res 2002;62:2791-2797 ©2002 by American Association for Cancer Research