Effects of EGCG on the anchorage-independent growth of EFT cells. Effects of EGCG on the anchorage-independent growth of EFT cells. A and B, EGCG impairs colony formation of TC32 and TC71 cells in soft agar. Cells were grown in soft agar for 14 d as described in Materials and Methods. After 14 d, plates were photographed (A) and the colonies were counted (B). Columns, representative of three replicate wells for each condition. Statistical analysis was done by Student's t test. C, effects of EGCG on the activation and expression of apoptotic and cell cycle regulatory proteins in TC32 and TC71 cells and HBMECs. TC32, TC71, and HBMEC cells were grown for 24 h and then treated with EGCG (50 and 100 μmol/L) for 12 h. Cells were lysed and subjected to immunoblotting with antibodies to the indicated proapoptotic and antiapoptotic proteins. β-Actin was used as a loading control. Hyung-Gyoo Kang et al. Mol Cancer Ther 2010;9:1396-1407 ©2010 by American Association for Cancer Research