Enzyme activity of PE-GA/Pt tuned by changing temperature or NIR light

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Fig. 2 Enzyme activity of PE-GA/Pt tuned by changing temperature or NIR light. Enzyme activity of PE-GA/Pt tuned by changing temperature or NIR light. (A) Illustration shows NIR light turns on the activity of PE-GA/Pt. PE-GA/Pt aggregates are disassembled upon NIR irradiation and recover the enzyme activity of digesting starch (the polysaccharide shown in black). Iodine (I3−, shown in orange) was added into the wells to stain the remaining starch, yielding blue-colored products. (B) Glucose production of PE-GA/Pt incubated at different temperatures for 30 min and the corresponding fold increase of enzyme activity. ***P < 0.001 analyzed by Student’s t test (n = 3). (C) Temperature changes of PE-GA and PE-GA/Pt solutions upon NIR irradiation (3.8 W cm−2, 30 min, n = 3). (D) Amount of glucose produced by PE-GA and PE-GA/Pt with or without NIR irradiation (3.8 W cm−2, 30 min). ***P < 0.001 analyzed by Student’s t test (n = 3). (E) Cycled off-on switching of the enzyme activity of PE-GA/Pt 2 by NIR light (3.8 W cm−2, 30 min for each cycle, n = 3). (F) Relative fold increase of enzyme activity of PE-GA/Pt 2 at different concentrations when heated at 25 and 45°C. Song Zhang et al. Sci Adv 2019;5:eaaw4252 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).