Frontal views of OBs from five 72 hpf embryos, as seen through the skull. Frontal views of OBs from five 72 hpf embryos, as seen through the skull. Glomeruli.

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Frontal views of OBs from five 72 hpf embryos, as seen through the skull. Frontal views of OBs from five 72 hpf embryos, as seen through the skull. Glomeruli labeled selectively with anti-calretinin (A1–A3, substacks of optical sections from same specimen), anti-Gαs/olf (B), anti-S100 (D), and anti-Gαo (E) antibodies. Four large and irregularly shaped regions (dG, dlG, maG, and vmG/vaG) were always labeled by anti-calretinin and anti-Gαs/olf antibodies (C,F). A1–A3, C, lG labeled only with the anti-calretinin antibody. The mdG2 and mdG5 were selectively immunoreactive (IR) to anti-S100 (D) and anti-Gαo (E), respectively, and displayed inconsistent staining with the anti-calretinin antibody (compare A1–C). The remaining mdG were labeled only with general structural markers, such as anti-SV2. F, The schematic summarizes the distributions of glomeruli at 72 hpf. The scale bar in A3 also applies to A1, A2. Oliver R. Braubach et al. J. Neurosci. 2013;33:6905-6916 ©2013 by Society for Neuroscience