Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.

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Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. A, immunocytochemistry for stable control (EGFP; a–c) and Panx1-EGFP (d–f) transfectants. Expression is shown by GFP fluorescence (a and d); Golgi apparatus and plasma membrane (b and e) were stained with WGA. Nuclei are visualized by DAPI in merged images (c and f). Panx1-EGFP seemed to localize to the perinuclear region (arrowheads) and cell membrane (arrows). Bar, 30 μm. B, Western blot analysis showing that Panx1-EGFP is predominantly associated with the membrane fraction. Whereas EGFP expression of the control transfectant was identified in the cytosol fraction (C), Panx1-EGFP was only detected in membrane-enriched fractions (M). Probing with anti-GAPDH antibody showed traces of GAPDH in the membrane-enriched fractions when compared with the cytosol fractions. Charles P.K. Lai et al. Cancer Res 2007;67:1545-1554 ©2007 by American Association for Cancer Research