Isolation of stable XPAKD and XPCKD HeLa clones.

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Isolation of stable XPAKD and XPCKD HeLa clones. Isolation of stable XPAKD and XPCKD HeLa clones. A. Western blot analysis of four XPCKD clones. M, mock-treated cells; C1, control vector (pBD650); C2, empty vector (pBD631). B. Immunocytochemical detection of XPC protein in two XPCKD clones, in XP44RO cells and in the XPC-complemented XP44RO cells. Magnification, ×350. C. Immunocytochemical detection of XPA and HSAkin17 in a XPAKD population 13 days after transfection. Magnification, ×350. D. Western blot analysis of 12 stable XPAKD clones (48 days in culture) and 2 stable XPCKD clones (113 days in culture). Denis S.F. Biard et al. Mol Cancer Res 2005;3:519-529 ©2005 by American Association for Cancer Research