Carcinoma cell–secreted IL-1 induces PGE2 production in MSCs

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Carcinoma cell–secreted IL-1 induces PGE2 production in MSCs Carcinoma cell–secreted IL-1 induces PGE2 production in MSCs. A, PGE2 (a) and COX-2 (b) were measured in the indicated conditioned medium or cultures. Carcinoma cell–secreted IL-1 induces PGE2 production in MSCs. A, PGE2 (a) and COX-2 (b) were measured in the indicated conditioned medium or cultures. PGE2 data are means ± SE, n = 3. ***, P < 0.005 (vs. that in LoVo medium). LoVo + MSC; LoVo lysate and MSC lysate mixed in equal amounts; LoVo/MSC, lysate of LoVo/MSC coculture. The same nomenclature applies to the SW1116 cells. B, a, MSCs were treated with LoVoCM, LoVoCM with neutralizing antibodies (500 ng/mL), or IL-1 only. b, MSCs were cocultured with LoVo cells expressing shRNAs against IL1α + IL1β (LoVoshIL1αβ-1, LoVoshIL1αβ-2), IL1β (LoVoshIL1β-1, LoVoshIL1β-2), or a scrambled sequence (LoVoshsc).c, MSCs were treated with DME, LoVoCM, or LoVoCM+IL-1ra. After incubation, media were collected and assayed for PGE2. Data are means ± SE, n = 3. C, a, IL-1α, IL-1β, and IL-1ra protein levels in conditioned medium of the carcinoma cells. Data are means ± SE, n = 3. b, PGE2 levels in carcinoma cells, MSCs (M), and cocultures of the carcinoma cells with MSCs. Data are means ± SE, n = 3. ***, P < 0.005 (vs. that in MSC culture). Hua-Jung Li et al. Cancer Discovery 2012;2:840-855 ©2012 by American Association for Cancer Research