Generation of an HPV–human PPI map. Generation of an HPV–human PPI map. A, Representation of the HPV genome, as well as the individual viral proteins used in this study (to scale), colored according to their canonical function during the viral replication cycle. B, Summary of the workflow used to construct the PPI network. C, Receiver operating characteristic curve illustrating MiST prediction accuracy of a set of 27 gold-standard interactions (see Supplementary Table S1) with the curve inflection point marked with a red “×.” The threshold of MiST 0.75 recalled 18 gold-standard interactions and a total of 137 high-confidence interactions (see also Supplementary Table S2). D, Number of human proteins interacting with each individual viral protein. E and F, Heat maps representing manually curated enriched cellular components (E) and biological pathways and processes (F) of human proteins interacting with individual HPV bait proteins (see also Supplementary Table S3). Colors represent statistical significance as indicated by the respective color scale. Manon Eckhardt et al. Cancer Discov 2018;8:1474-1489 ©2018 by American Association for Cancer Research