Illustration of optogenetic cell stimulation with OLED microarrays

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Date of download: 12/30/2017 Copyright © ASME. All rights reserved.

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From: Magnetic Field Effects on Laser Drilling

Volume 16, Issue 8, Pages (August 2016)

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Presentation transcript:

Fig. 1 Illustration of optogenetic cell stimulation with OLED microarrays. Illustration of optogenetic cell stimulation with OLED microarrays. (A) Schematic of OLED microarray with cells adhered on top of the array (not drawn to scale). The microarray is connected to a high-definition multimedia interface (HDMI) driver with a flexible connector. Each pixel of the array can be turned on and off by the driver and the CMOS backplane, thus providing controlled light exposure of individual cells. Light-induced changes in cell membrane current are measured with a patch clamp electrode (voltage clamp mode, whole-cell configuration). The cross section on the right shows the layer structure of the OLED array. (B) Optical power density at the surface of the OLED microarray versus applied cathode voltage. (C) Picture of the experimental setup with an upright microscope equipped with water immersion objective used to direct positioning of the patch electrode housed in a glass pipette. The microarray and the ground electrode are placed in a petri dish filled with salt solution. The flexible connector links the array to the driver located outside the field of view. A flow system constantly renews the salt solution. Anja Steude et al. Sci Adv 2016;2:e1600061 Copyright © 2016, The Authors