Use of the caGFP reporter gene to monitor tetracycline-induced gene expression in C. albicans yeast, hyphal, and opaque cells. Use of the caGFP reporter.

Slides:

Advertisements

Similar presentations

Figure S1 – The ammount of depleted glutathione is similar to the ammount of extracellular glutathione after staurosporine (STS) treament. The graph shows.

Advertisements

BY4742  yhr087w YPDYP25YPD KCl 1MYP30 S.1 Defects of the YHR087W deletion mutant under osmotic stress conditions. Growth of the BY4742 and  yhr087w strains.

C H-NS (nM) 14 nM FIS28 nM FIS56 nM FISno FIS Figure S1. FIS and H-NS can simultaneously interact with cspA promoter.

Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1 Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,

Volume 20, Issue 4, Pages (April 2013)

Sgs1Δ103−322 physically interacts with Top3–Rmi1.

Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation Stefan W. Stoll, Philip E. Stuart, Sylviane Lambert, Alberto.

Induction of apoptosis by CDIP expression.

HIV-Protease Inhibitors Reduce Cell Adherence of Candida Albicans Strains by Inhibition of Yeast Secreted Aspartic Proteases Margarete Borg-von Zepelin,

Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,

MEC1-Dependent Redistribution of the Sir3 Silencing Protein from Telomeres to DNA Double-Strand Breaks Kevin D Mills, David A Sinclair, Leonard Guarente

Volume 25, Issue 12, Pages (June 2015)

Qian Yang, Atsushi Yamada, Shioko Kimura, Jeffrey M. Peters, Frank J

Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1 Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,

Volume 11, Issue 6, Pages (June 2003)

Stefan W. Stoll, Jessica L. Johnson, Yong Li, Laure Rittié, James T

Biofilm Inhibitors that Target Amyloid Proteins

Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ mpk1Δ, and khd1Δ ccr4Δ mpk1Δ mutant strains. Rom2 protein levels in the ccr4Δ, khd1Δ ccr4Δ, ccr4Δ.

Hery Ratsima, Diego Serrano, Mirela Pascariu, Damien D’Amours

Dibutyryl cAMP does not rescue staurosporine-induced filamentation of a cyr1Δ/cyr1Δ mutant. Dibutyryl cAMP does not rescue staurosporine-induced filamentation.

Supplementary Figure 1 RT-PCR BclxL mismatch sc35 Dox BclxS

MEC1-Dependent Redistribution of the Sir3 Silencing Protein from Telomeres to DNA Double-Strand Breaks Kevin D Mills, David A Sinclair, Leonard Guarente

The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches Dirk Winter, Alexandre.

(A) Growth curves of strains at 30°C in SPP medium or SPP supplemented with 15 μM paclitaxel or 10 μM oryzalin. (A) Growth curves of strains at 30°C in.

Volume 91, Issue 4, Pages (November 1997)

Crucial Role for Membrane Fluidity in Proliferation of Primitive Cells

Immunofluorescece analysis of c-src and v-src distribution in wild-type yeast cells. Immunofluorescece analysis of c-src and v-src distribution in wild-type.

White-Opaque Switching in Candida albicans Is Controlled by Mating-Type Locus Homeodomain Proteins and Allows Efficient Mating Mathew G. Miller, Alexander.

The Cdk-Activating Kinase (CAK) from Budding Yeast

Volume 20, Issue 4, Pages (April 2013)

Mutants impaired in N-acetylglucosamine transport and catabolism display morphological and pH neutralization defects within macrophages. Mutants impaired.

Volume 55, Issue 4, Pages (April 1999)

Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1 Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,

High Invasive Melanoma Cells Induce Matrix Metalloproteinase-1 Synthesis in Fibroblasts by Interleukin-1α and Basic Fibroblast Growth Factor-Mediated.

Control of Cell Morphogenesis in Bacteria

Staurosporine-induced filamentation requires Cyr1 and PKA

Gap2 is required for growth on phenylalanine as the sole source of nitrogen. Gap2 is required for growth on phenylalanine as the sole source of nitrogen.

Legionella Reveal Dendritic Cell Functions that Facilitate Selection of Antigens for MHC Class II Presentation Annie L Neild, Craig R Roy Immunity

ERMES impacts on the exposure of pathogen-associated molecular patterns during hyphal growth. ERMES impacts on the exposure of pathogen-associated molecular.

MMV is a novel antifungal agent.

Volume 17, Issue 1, Pages (January 2005)

Volume 135, Issue 6, Pages (December 2008)

Severe Oxidative Stress Causes Inactivation of DnaK and Activation of the Redox- Regulated Chaperone Hsp33 Jeannette Winter, Katrin Linke, Anna Jatzek,

Volume 56, Issue 2, Pages (August 1999)

Identification of an Asymmetrically Localized Determinant, Ash1p, Required for Lineage- Specific Transcription of the Yeast HO Gene Anita Sil, Ira Herskowitz

The UCS Domain Protein She4p Binds to Myosin Motor Domains and Is Essential for Class I and Class V Myosin Function Stefanie Wesche, Marc Arnold, Ralf-Peter.

Physcomitrella patens Auxin-Resistant Mutants Affect Conserved Elements of an Auxin- Signaling Pathway Michael J. Prigge, Meirav Lavy, Neil W. Ashton,

Volume 23, Issue 8, Pages (May 2018)

Differential Responses of S100A2 to Oxidative Stress and Increased Intracellular Calcium in Normal, Immortalized, and Malignant Human Keratinocytes Tong.

Paul B. Mason, Kevin Struhl Molecular Cell

Irina Chernyakov, Felipe Santiago-Tirado, Anthony Bretscher

RIF1 interacts with protein phosphatase 1 isoforms

Protein Dimerization Generates Bistability in Positive Feedback Loops

Volume 3, Issue 5, Pages (May 2001)

Keratinocyte Apoptosis Induced by Ultraviolet B Radiation and CD95 Ligation – Differential Protection through Epidermal Growth Factor Receptor Activation.

Depletion of COPI protein inhibits cis to trans cisternal maturation.

Nuclear localization of Sfl1.

Susceptibility to hydrogen peroxide.

Using CRISPRi to knock down acm2 expression in L. plantarum WCFS1.

CozE homologs do not dramatically affect cell division in L. plantarum

dCas9-mediated repression of transcription.

Expression of ylxR-containing operon revealed by lacZ and gfp fusion analysis. Expression of ylxR-containing operon revealed by lacZ and gfp fusion analysis.

C-MYC overexpression or growth in serum-containing media induces EMT in HBEC3p53,KRAS. c-MYC overexpression or growth in serum-containing media induces.

C. neoformans crosses the HCMEC/D3 cell line, an in vitro model of the BBB. (A) The in vitro BBB model consists of a transwell apparatus separating the.

Establishment of HeLa/rtTAA/TRE-N1-IC cell line.

Cell death induced by HET domain expression.

Keratin Degradation by Dermatophytes Relies on Cysteine Dioxygenase and a Sulfite Efflux Pump Maria Grumbt, Michel Monod, Tsuyoshi Yamada, Christian.

Volume 22, Issue 6, Pages (March 2012)

Microscopic imaging of different C

Identification of bacterial species producing the anti-Candida molecules. Identification of bacterial species producing the anti-Candida molecules. (A)

Presentation transcript:

Use of the caGFP reporter gene to monitor tetracycline-induced gene expression in C. albicans yeast, hyphal, and opaque cells. Use of the caGFP reporter gene to monitor tetracycline-induced gene expression in C. albicans yeast, hyphal, and opaque cells. The figure shows phase-contrast (upper panels) and corresponding fluorescence (lower panels) micrographs of the cells. In all conditions, the two independently constructed strains of each pair (A and B) behaved identically, and only one of them is shown. (A) Strains TETG25A and -B and the control strain SC5314 were grown at 30°C in YPD medium in the absence (−Dox) or presence (+Dox) of 50 μg ml−1 doxycycline. Photos were taken at the indicated time points. (B) Hyphal growth of strains TETG25A and -B and SC5314 was induced by incubating the cells in RPMI medium with 10% FCS at 37°C without or with 50 μg ml−1 doxycycline as indicated. (C) Opaque cells of strains WTETG40A and -B and the control strain WO-1 were grown in Lee's medium at 25°C in the absence or presence of 50 μg ml−1 doxycycline and photos were taken at the indicated time points. Yang-Nim Park, and Joachim Morschhäuser Eukaryotic Cell 2005; doi:10.1128/EC.4.8.1328-1342.2005