Badger Connect Inaugural Research Services Fair Workshop

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Badger Connect Inaugural Research Services Fair Workshop Thursday, May 3, 2018 Human Cell Line Authentication William M. Rehrauer, PhD Associate Professor, UWSMPH Department of Pathology Co-Director of the Translational Research Initiatives in Pathology (TRIP) Lab

TRIP: Human Cell Line Authentication Service Sample Types Accepted DNA Cells Maximum Turnaround Time 10 working days Cost $125 DNA sample $135 Cell sample Interpretative Report Identification Authentication Contamination (2% Sensitivity) Analyses relative to: Publication(s) Repository(s) Baseline Passage Monitoring PowerPlex® 16 HS System CSF1PO D5S818 D21S11 PENTA_D TH01 TPOX D13S317 D7S820 D18S51 D16S539 D8S1179 D3S1358 FGA VWA AMEL PENTA_E Analysis of 15 Microsatellite (STR) Loci and Amelogenin (Gender Specific Loci)

TRIP: Human Cell Line Authentication Service PowerPlex® 16 HS System CSF1PO D5S818 D21S11 PENTA_D TH01 TPOX D13S317 D7S820 D18S51 D16S539 D8S1179 D3S1358 FGA VWA AMEL PENTA_E Analysis of 15 Microsatellite (STR) Loci and Amelogenin (Gender Specific Loci) Total (2014-2017): 1,705 UW Submissions: 2.5% potentially contaminated 26.3% <55% match (misidentified)