HES1-dependent activation of SRC/STAT3 pathway is mediated by transcription-independent mechanism. HES1-dependent activation of SRC/STAT3 pathway is mediated.

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HES1-dependent activation of SRC/STAT3 pathway is mediated by transcription-independent mechanism. HES1-dependent activation of SRC/STAT3 pathway is mediated by transcription-independent mechanism. A. TSA does not affect HES1-dependent STAT3 activation. HeLa/rtTAA/TRE-HES1 cells were treated with 1 μg/mL doxycycline and 20 nmol/L of TSA for the stated amount of time. Cells were examined for cellular level of STAT3, phosphorylated STAT3 at Tyr 705, or HES1 by immunoblot analysis. B. HES1 transcriptional regulation function is irrelevant to HES1-mediated activation of SRC/STAT3 pathway. HeLa/rtTAA/TRE-N1-IC/dnHES1 or HeLa/rtTAA/TRE-HES1/dnHES1 cell line stably expressing dominant negative HES1 (dnHES1) was established. After induction of Notch1-IC or HES1 by doxycycline (1 μg/mL) treatment for 48 h, cellular level of STAT3 or phosphorylated STAT3 at Tyr 705 was analyzed by immunoblotting in respective cell lines. Jae Ho Lee et al. Mol Cancer Res 2009;7:1663-1671 ©2009 by American Association for Cancer Research