Structures of wild-type and mutated recombinant CDX2.

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Structures of wild-type and mutated recombinant CDX2. Structures of wild-type and mutated recombinant CDX2. A, the nucleotide and amino acid sequences of wild-type and frameshift-mutated CDX2. The frameshift mutation caused by one G insertion in the coding 7 G microsatellite of HNP-8 tumor resulted in substitution with tumor-specific peptide consisting of 30 amino acids. B, the structures of recombinant wild-type and mutated CDX2 fusion proteins used in this study. MBP was fused at the NH2 terminus of the recombinant proteins. a, recombinant wild-type CDX2 and the fragment; b, recombinant mutated CDX2 and 2 fragments. Bold lines, normal amino acid sequences shared with wild-type CDX2. Open box, eight guanidine repeats in the coding region. Hatched domain, tumor-specific COOH-terminal 30 amino acids caused by the frameshift mutation. Toshiaki Ishikawa et al. Cancer Res 2003;63:5564-5572 ©2003 by American Association for Cancer Research