BCL6 represses NOTCH2 complex genes and NOTCH activity.

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BCL6 represses NOTCH2 complex genes and NOTCH activity. BCL6 represses NOTCH2 complex genes and NOTCH activity. A, The relative transcript abundance of NOTCH2, MAML1, MAML2, and RBPJ was examined by qPCR in DoHH2 (black bars) and Sc-1 (gray bars) 72 hours after BCL6 siRNA depletion versus control siRNA. Values were normalized to HPRT, and fold change (y-axis) is represented over a scrambled siRNA control. B, The relative transcript abundance of NOTCH2, MAML1, MAML2, and RBPJ was examined by qPCR in FL cell lines DoHH2 (black bars) and Sc-1 (gray bars) 72 hours after RI-BPI treatment (15 μmol/L RI-BPI) on the right. Values were normalized to HPRT, and fold change (y-axis) is represented over control peptide (CP). C, Reporter assays performed in DoHH2 (black bars) and Sc-1 (gray bars) cells transfected with pGL2-HESAB (NOTCH reporter) or pGL2 control vector, and with BCL6 (siBCL6) or control siRNA (siC). The y-axis shows the luciferase activity relative to renilla (internal control). All panels represent the mean of three independent experiments, each performed in triplicate; error bars, SEM. The statistical values are based on an unpaired two-tailed t test. ***, P < 0.0001. D, Expression values (FPKM) of NOTCH ligands DLL1, DLL3, DLL4, JAG1, and JAG2 on DoHH2 (blue), Sc-1 (yellow), OCI-Ly1 (purple), SU-DHL-4 (green). E, As in D, expression values (FPKM) of metalloproteases ADAM10 and ADAM17 on the same cell lines. F, Expression values (FPKM) of DLL1 (top), DLL3 (middle), and DLL4 (bottom) from left to right: naïve B cells (NB; n = 5), GC B cells (GCB; n = 4), centroblast (CB; n = 7), centrocytes (CC; n = 0.7), bone marrow plasma cells (BMPC; n = 3), tonsillar plasma cells (TPC; n = 5), memory B cells (MB; n = 8), and FL (n = 77) from independent specimens of each. *, P ≤ 0.05. Square dots are outliers (below the first quartile or above the fourth quartile). G, As in F, expression values (FPKM) of JAG1 (top) and JAG2 (bottom). H, As in G, expression values (FPKM) of ADAM10 (top) and ADAM 17 (bottom). I, Flow cytometry to assess apoptosis of FL cell lines driven by DLL1 ligand. The percentage of apoptotic cells is observed on the upper-right quadrant of double-positive labeled cells for propidium iodide (PI; y-axis) and Annexin V (x-axis) on DoHH2 (top) and Sc-1 (bottom) cell lines cocultured with HS5-control (right graph) or HS5-DLL1 (triplicate in left graphs) stromal cell line for 48 hours. See Supplementary Fig. S5 for additional data. Ester Valls et al. Cancer Discov 2017;7:506-521 ©2017 by American Association for Cancer Research