Hydroxychloroquine preferentially induces apoptosis of CD45RO+ effector T cells by inhibiting autophagy: A possible mechanism for therapeutic modulation.

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Presentation transcript:

Hydroxychloroquine preferentially induces apoptosis of CD45RO+ effector T cells by inhibiting autophagy: A possible mechanism for therapeutic modulation of T cells Jorg van Loosdregt, PhD, Roberto Spreafico, PhD, Maura Rossetti, PhD, Berent J. Prakken, MD, PhD, Martin Lotz, MD, PhD, Salvatore Albani, MD, PhD Journal of Allergy and Clinical Immunology Volume 131, Issue 5, Pages 1443-1446.e1 (May 2013) DOI: 10.1016/j.jaci.2013.02.026 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 HCQ impairs autophagy in CD4+ T cells. A, Autophagy in human CD4+ cells, cultured in the presence of HCQ, n = 8. B, Histograms of Fig 1, A. C, Autophagosome visualization in CD4+ cells, cultured with 20 μmol/L of HCQ. D, Quantification of Fig 1, C, n = 3. E, p62, LC3, and ß-actin expression in CD4+ cells, n = 4. F, Autophagy in human CD4+CD45RA+CD45RO− and CD4+CD45RA−CD45RO+ cells, cultured with HCQ, n = 7. MFI, Mean fluorescent intensity. *P < .05. Journal of Allergy and Clinical Immunology 2013 131, 1443-1446.e1DOI: (10.1016/j.jaci.2013.02.026) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 HCQ treatment preferentially induces apoptosis in CD4+CD45RA−CD45RO+ T cells. A, Apoptosis (by Annexin V staining) in human CD4+ cells, cultured in the presence of HCQ, n = 8. B, Histograms of Fig 2, A. C, Apoptosis in human CD4+CD45RA+CD45RO− and CD4+CD45RA−CD45RO+ cells, n = 7. D, FACS plot of HCQ-treated CD4+CD45RA+CD45RO− (blue) and CD4+CD45RA−CD45RO+ (red) cells stained with propidium iodide (PI). E and F, Sorted CD4+CD45RA+ (CFSE) or CD4+CD45RO+ (EF670) cells were mixed at a 1:1 ratio and cultured in the presence of HCQ (n = 4). CFSE, Carboxyfluorescein succinimidyl ester; FACS, fluorescence-activated cell sorting. ∗P < .05. Journal of Allergy and Clinical Immunology 2013 131, 1443-1446.e1DOI: (10.1016/j.jaci.2013.02.026) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions