Fig. 4 Comparing dynamics of colloids bound to the interface and free in solution. Comparing dynamics of colloids bound to the interface and free in solution.

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Fig. 4 Comparing dynamics of colloids bound to the interface and free in solution. Comparing dynamics of colloids bound to the interface and free in solution. (A and B) Mean square displacement versus delay time for 1.2-μm colloids diffusing freely in solution (A) and for DNA-bound colloids diffusing on the OD surfaces averaged over five tracks (B). The inset shows a typical single-particle track. (C) Microscope image of 0.57-μm colloids bound to an OD surface, focusing on its south pole. The square selection was used for DDM analysis. (D) Decay time τ (q) as a function of the scattering vector q extracted from DDM analysis for both colloids diffusing on OD surfaces [as shown in (C)] and from microscope movies taken from free colloids in solution; the theoretical line passing through the data for the free colloids was obtained, assuming a colloid diameter of 0.57 μm, a buffer solution viscosity of 0.9 mPa s, and room temperature. Darshana Joshi et al. Sci Adv 2016;2:e1600881 Copyright © 2016, The Authors