Limited PD-1 expression in normal human tissues.

Slides:

Advertisements

Similar presentations

Patient #1 Patient #2 Patient #3 Normal Lung

Advertisements

Lymphocytes Cell Surface Markers

P52 Activation in Monomorphic B-Cell Posttransplant Lymphoproliferative Disorder/Diffuse Large B-Cell Lymphoma without BAFF-R Expression Alexis Proust,

Immunologic effects of F16-IL2 therapy.

FigS3 IHC analysis of Drosha and DGCR8 protein distribution in the hippocampus, frontal cortex and cerebellum of control cases. In the hippocampus both.

Table S2. Normal human tissue binding of FG88 mAbs.

Figure 2 Expression of complement activation products in renal samples

CD4+ICOS+IL-4+ TFH cells are sparse in human SLOs.

Histopathologic features (all images at ×10 magnification).

Figure 3 Immunohistochemical analyses of positive and negative Epstein-Barr virus (EBV) control tissues using immunostaining Immunohistochemical analyses.

BCL-6 Protein Expression in Human Peripheral T-Cell Neoplasms Is Restricted to CD30+ Anaplastic Large-Cell Lymphomas by Antonino Carbone, Annunziata Gloghini,

Immunohistochemistry of CD8 (A and B) and PD-L1 (C–F).

IHC staining of FFPE esophageal tissues in tissue microarrays (×20).

Programmed cell death ligand-1 (PD-L1) expression of alveolar macrophages. Programmed cell death ligand-1 (PD-L1) expression of alveolar macrophages. (A)

The absence of ADCC by nivolumab in vitro.

Tregs and APC subsets in TDLNs of patients with cervical cancer.

Examples of cancer tissue profiles.

Examples of normal tissue profiles.

The summary page for cancer profiles.

Tetramer staining performed to determine frequencies of epitope-specific T cells after clearance of HPV16 infection but prior to isolation of T-cell clones.

Programmed cell death ligand-1 (PD-L1) immunohistochemistry for pulmonary adenocarcinoma tissues. Programmed cell death ligand-1 (PD-L1) immunohistochemistry.

MYC DNA is structurally abnormal and MYC protein is overexpressed in MCL cells. MYC DNA is structurally abnormal and MYC protein is overexpressed in MCL.

NICD1 immunohistochemical staining in normal and tumor tissue.

Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice. Secondary storage of GM2 ganglioside and cholesterol in Fuca1-deficient mice.

PD-1 blockade enhances T-cell function.

SIRT1 is downregulated in human gastric cancer (GC).

IGF1R is present in endometrial luminal epithelium at the receptive phase. IGF1R is present in endometrial luminal epithelium at the receptive phase. Endometrial.

Analysis of tumor-infiltrating CD8+ T cells and effect of T and NK cell depletion. Analysis of tumor-infiltrating CD8+ T cells and effect of T and NK cell.

Histopathology and bcl-2 immunohistochemistry of in situ follicular lymphoma.(A-B) Low-power view of H&E-stained (A) and bcl-2–stained (B) sections showing.

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

Activating mutation of Ras is associated with CDCP1 expression in NSCLC. A, NSCLC cell lines with Ras + B-Raf mutations and wild-type Ras were examined.

Immunohistochemical analyses of SHH expression levels in nonmalignant prostate and adenocarcinoma tissues. Immunohistochemical analyses of SHH expression.

The expression of SPINDLIN1 in cancer tissues.

Geographic colocalization of PD-L1+ tumor cells with infiltrating immune cells in MCC. The predominant pattern of tumor cell PD-L1 expression is at the.

Expression profile of RSPO1 and 2 in gastric cancer.

FAK overexpression in invasive human breast cancer and DCIS

B7-1 and PD-1 compete for binding to PD-L1.

FAK overexpression in invasive human colon cancer and colon dysplasia.

Progastrin expression in colonic tissues and Kaplan–Meier survival curves. Progastrin expression in colonic tissues and Kaplan–Meier survival curves. A,

PD-L1 expression by melanocytes is observed in geographic association with TILs but does not depend on BRAF V600E. PD-L1 expression by melanocytes is observed.

IL6 mRNA is not detected in metastatic prostate cancer cells.

PD-1 inhibition stimulates the proliferation and cytokine secretion of exhausted/senescent CD8+ T cells in vitro. PD-1 inhibition stimulates the proliferation.

IL-13Rα2 expression is associated to human colon cancer progression and poor survival of patients with colorectal cancer. IL-13Rα2 expression is associated.

Melanoma patient monocytes have altered expression of inflammatory and surface markers. Melanoma patient monocytes have altered expression of inflammatory.

SAF-1 expression in clinical breast cancer tissues.

FILM immunohistochemical protein signature is significantly associated with poor survival. FILM immunohistochemical protein signature is significantly.

Epithelial-derived cancer cells and tumor-residing APCs have higher HLA-E expression but reduced MHC-1a expression. Epithelial-derived cancer cells and.

Immunohistochemical staining of primary and metastatic prostatic carcinomas with anticyclin D1 and anti-Ki67 monoclonal antibodies. Immunohistochemical.

IL1R8 is downmodulated in human lymphoma cell lines.

Effect of nivolumab or ipilimumab on immune responses to vaccination in cynomolgus monkeys. Effect of nivolumab or ipilimumab on immune responses to vaccination.

Representative photograph of somatic mutations detected by PCR-SSCP.

Reduced klotho expression in pancreatic cancer.

PD-1 expression in ovarian cancer.

PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate distinct mechanisms of regulation. PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate.

ODC protein expression in paired benign and cancer tissue obtained from patients with PCA. The protein expression was measured by immunoblot analysis in.

Progastrin staining in colon adenomas and adenocarcinomas.

Histology (H&E; original magnification, ×100 for B-2P/C-12P/C-64P and ×40 for C-10P; top) of small-sized lung adenocarcinomas and immunohistochemical staining.

Specific reaction with MIB1, M30, and CD31 monoclonal antibodies in primary human cervical carcinoma before (A, C, and E) and after (B, D, and F) Tam treatment.

Tumor-specific panel of mAbs are reactive with human B7-H3.

Amino acid residues in the PD-L1 IgV domain involved in PD-1 and/or B7-1 binding. Amino acid residues in the PD-L1 IgV domain involved in PD-1 and/or B7-1.

Reduced expression of the tumor suppressor PTEN occurs in tumors with both squamous cell and adenocarcinoma histology. Reduced expression of the tumor.

Vorozole treatment-associated changes in protein abundance of multiple antigens. Vorozole treatment-associated changes in protein abundance of multiple.

Immunohistochemical determination of TIM-3 expression in ccRCC.

REV-ERBα deficiency reduces frequency and number of NKp46+ ILC3s.

Expression of the angiogenic phenotype in the 4-NQO mouse model of HNSCC. A, tissue sections containing areas of normal, hyperkeratosis, dysplasia, and.

PEGPH20 depletes HA and decompresses intratumoral vessels.

The inverse correlation between PARP1 and PD-L1, PAR, and p-STAT3 expression in surgical specimens of ovarian cancer. The inverse correlation between PARP1.

RIL21 and checkpoint blockade restore IFNγ production in Tim-3+PD-1+ intratumoral NK cells from cancer patients. rIL21 and checkpoint blockade restore.

IHC staining of FFPE esophageal tissues in tissue microarrays (×20).

Expression of CAR, endogenous TCR, and target-cell receptors.

Presentation transcript:

Limited PD-1 expression in normal human tissues. Limited PD-1 expression in normal human tissues. Immunohistochemistry in positive control tissue, hyperplastic tonsil (A), using FITC-conjugated nivolumab. Strong immunoreactivity was distributed in subsets of lymphocytes primarily in germinal center of the tonsil; FITC-conjugated human IgG4 was used as an isotype control in tonsil (B). No specific staining was observed in cerebellum (C), heart (D), lung (E), or kidney (F). Positive staining was revealed in a very small number of scattered endocrine cells (G–I) in four of five pituitary samples. G and H represent two positive samples, and I represents negative pituitary tissue. Insets in G and H are high-power views showing strong immunoreactivity in large cytoplasmic spherical organelles, enigmatic body-like structures, with weak cytoplasmic staining. Br, bronchiole of the lung; GC, germinal center of the tonsil; Gl, glomerulus of the kidney; GL, granular layer of the cerebellum cortex; ML, molecular layer of the cerebellum cortex; MZ, mantle zone of the tonsil. Changyu Wang et al. Cancer Immunol Res 2014;2:846-856 ©2014 by American Association for Cancer Research