BHLHB2 is part of a protein–DNA complex associated with the proximal E-box element of Bdnf promoter 4. BHLHB2 is part of a protein–DNA complex associated.

Slides:

Advertisements

Similar presentations

Fig. 7 Localization of the element(s) responsible for the transcriptional suppression by PPAR-γ. A, Rat VSMCs were transfected with either −1969/+104-luc,

Advertisements

Figure 3. Activation of Wild-Type and Point-Mutated MMP-9 Promoter Constructs by IL-1β A, Schematic representation of the different 1.3-kb MMP-9-luciferase.

by Hanna S. Radomska, Anne B

by Kumar Kotlo, Douglas E. Hughes, Victoria L. M

Serotonin Transporter Promoter Gain-of-Function Genotypes Are Linked to Obsessive- Compulsive Disorder Xian-Zhang Hu, Robert H. Lipsky, Guanshan Zhu,

Novel Cis Element for Tissue-Specific Transcription of Rat Platelet-Derived Growth Factor β-Receptor Gene by Yasunori Takata, Yutaka Kitami, Tomikazu Fukuoka,

UVB Increases Urokinase-Type Plasminogen Activator Receptor (uPAR) Expression1 Christoph Marschall, Toshiko Nobutoh, Evelyn Braungart, Kathrin Douwes,

rs alters protein/DNA complex formation with the involvement of p300.

Characterization of the Human Platelet/Endothelial Cell Adhesion Molecule-1 Promoter: Identification of a GATA-2 Binding Element Required for Optimal Transcriptional.

Volume 126, Issue 1, Pages (January 2004)

The Role of Transcription Factor PU

by Hong Yin, and K. L. Blanchard

by Hong Hao, Huiling Qi, and Manohar Ratnam

Cooperative formation of a higher‐order complex containing AP1 and HMG‐I(Y). Cooperative formation of a higher‐order complex containing AP1 and HMG‐I(Y).

Promoter and 3′-Untranslated-Region Haplotypes in the Vitamin D Receptor Gene Predispose to Osteoporotic Fracture: The Rotterdam Study Yue Fang, Joyce.

DHEA(S) and Allo activated transcription factors NF-κB and CREB in serum-deprived PC12 cells. DHEA(S) and Allo activated transcription factors NF-κB and.

Naoko Kanda, Shinichi Watanabe Journal of Investigative Dermatology

Serotonin Transporter Promoter Gain-of-Function Genotypes Are Linked to Obsessive- Compulsive Disorder Xian-Zhang Hu, Robert H. Lipsky, Guanshan Zhu,

IFN-γ Upregulates Expression of the Mouse Complement C1rA Gene in Keratinocytes via IFN-Regulatory Factor-1 Sung June Byun, Ik-Soo Jeon, Hyangkyu Lee,

The interferon regulatory factor ICSBP/IRF-8 in combination with PU

Sp1 Is Required for Glucose-Induced Transcriptional Regulation of Mouse Vesicular Glutamate Transporter 2 Gene Tao Li, Liqun Bai, Jing Li, Suzu Igarashi,

Volume 54, Issue 1, Pages (July 1998)

Volume 21, Issue 1, Pages (July 2004)

Volume 68, Issue 3, Pages (September 2005)

Jason Park, Stephanie Schulz, Scott A. Waldman Gastroenterology

Qiujie Jiang, Yasushi Matsuzaki, Kehua Li, Jouni Uitto

Adam C Bell, Adam G West, Gary Felsenfeld Cell

a b AP-1 Sp1 Nuclear extract Competitor (100X) CDCA 50 µM + - M

Transcriptional Control of the Mouse Col7a1 Gene in Keratinocytes: Basal and Transforming Growth Factor-β Regulated Expression Michael Naso, Jouni Uitto,

Yin-Yang 1 Negatively Regulates the Differentiation-Specific Transcription of Mouse Loricrin Gene in Undifferentiated Keratinocytes Xuezhu Xu, Yasuhiro.

Androgen Regulation of the Human Hair Follicle: The Type I Hair Keratin hHa7 Is a Direct Target Gene in Trichocytes Luis F. Jave-suarez, Lutz Langbein,

Inhibition of nuclear factor-κB activation reduces cortical tubulointerstitial injury in proteinuric rats Gopala K. Rangan, Yiping Wang, Yuet-Ching Tay,

Volume 62, Issue 3, Pages (September 2002)

Volume 57, Issue 5, Pages (May 2000)

Volume 20, Issue 4, Pages (April 1998)

Xiangmei Chen, Wenhu Liu, Jianzhong Wang, Xiaodan Wang, Zhiheng Yu

Leslie A. Bruggeman, Scott H. Adler, Paul E. Klotman

Volume 8, Issue 2, Pages (February 1998)

In situ non-radioactive detection of nuclear factors in paraffin sections by Southwestern histochemistry Miguel Angel Hernández-Presa, Carmen Gómez-Guerrero,

DNA binding of activator protein-1 is increased in human mesangial cells cultured in high glucose concentrations William A. Wilmer, Fernando G. Cosio

Interleukin-6-Resistant Melanoma Cells Exhibit Reduced Activation of STAT3 and Lack of Inhibition of Cyclin E-Associated Kinase Activity Markus Böhm,

The G/G Genotype of a Resistin Single-Nucleotide Polymorphism at −420 Increases Type 2 Diabetes Mellitus Susceptibility by Inducing Promoter Activity.

Regulation of the Melanoma Cell Adhesion Molecule Gene in Melanoma: Modulation of mRNA Synthesis by Cyclic Adenosine Monophosphate, Phorbol Ester, and.

STAT proteins mediate angiotensin II–induced production of TIMP-1 in human proximal tubular epithelial cells Xiangmei Chen, Jianzhong Wang, Feng Zhou,

Identification of Rare, Disease-Associated Variants in the Promoter Region of the RNF114 Psoriasis Susceptibility Gene Alexandros Onoufriadis, Michael.

The α and η Isoforms of Protein Kinase C Stimulate Transcription of Human Involucrin Gene Hidetoshi Takahashi, Kazuhiro Asano, Akira Manabe, Motoshi.

IFN-γ Represses IL-4 Expression via IRF-1 and IRF-2

John W. Haycock, Mark Wagner, Sheila Mac Neil

Volume 10, Issue 19, Pages (October 2000)

Defining the Regulatory Elements in the Proximal Promoter of ΔNp63 in Keratinocytes: Potential Roles for Sp1/Sp3, NF-Y, and p63 Rose-Anne Romano, Barbara.

FoxB protein binds directly to the cis-regulatory element of the Bra gene. FoxB protein binds directly to the cis-regulatory element of the Bra gene. (A)

TNF Regulates the In Vivo Occupancy of Both Distal and Proximal Regulatory Regions of the MCP-1/JE Gene Dongsheng Ping, Peter L. Jones, Jeremy M. Boss

Electrophoretic mobility shift assays for NF-κB in BRP (A) and BREC (B). Electrophoretic mobility shift assays for NF-κB in BRP (A) and BREC (B). Confluent.

Expression of SENP2 mRNA is regulated by palmitate-induced NF-κB activation. Expression of SENP2 mRNA is regulated by palmitate-induced NF-κB activation.

The recombinant Puf4 pumilio domain (Puf4-PD) binds to PUF consensus elements in HXL1 mRNA. The recombinant Puf4 pumilio domain (Puf4-PD) binds to PUF.

Bart A. Jessen, Marjorie A. Phillips, Robert H. Rice

Supershift assay of the Pax4 are shown.

The Vitamin D Response Element of the Involucrin Gene Mediates its Regulation by 1,25-Dihydroxyvitamin D3 Daniel D. Bikle, Dean Ng, Yuko Oda, Karen Hanley,

NF-Y binds the −4,425/−4,392 G6PC2 promoter region in vitro.

ERF12 Binds Directly to the DRE/CRT Element in the DOG1 Promoter.

Interneurons originate outside the Emx1 lineage

Interaction of MAPJD with E-box sequence of the RIOK1 gene.

NF-kB binds to the NF-κB binding site immediately adjacent to the AT-rich regulatory region. NF-kB binds to the NF-κB binding site immediately adjacent.

Proximal E-box sequence in Bdnf promoter 4 functions as a transcriptional suppressor. Proximal E-box sequence in Bdnf promoter 4 functions as a transcriptional.

Basal and NMDA receptor-dependent changes in reporter gene activity for Bdnf promoter deletions. Basal and NMDA receptor-dependent changes in reporter.

Volume 16, Issue 5, Pages (December 2004)

Characterization of the complex formed at the Fra-1 RCE1.

A, gel retardation assay in colon cancer cell line SW480.

Interaction of Cdx1 and Cdx2 with the putative Cdx-binding sites of the human DSC2 promoter. Interaction of Cdx1 and Cdx2 with the putative Cdx-binding.

Stéphane Karlen, Lasse R. Braathen

Presentation transcript:

BHLHB2 is part of a protein–DNA complex associated with the proximal E-box element of Bdnf promoter 4. BHLHB2 is part of a protein–DNA complex associated with the proximal E-box element of Bdnf promoter 4. Autoradiograph showing a specific band indicative of a DNA–protein binding complex in NIH3T3 cell nuclear extracts to a 33P-labeled double-stranded DNA probe based on the 22 bp rat Bdnf promoter 4 IS region (−110 to −87). Addition of unlabeled competitor DNA caused the binding activity to disappear in a concentration-dependent manner. Other consensus E-box containing competitor DNAs were also effective in eliminating the specific band. When a polyclonal BHLHB2 peptide antibody was incubated with the DNA–protein complex, the migration of the specific band was further retarded (shown by the arrow labeled “supershift”). No supershift was observed when the DNA–protein complex was incubated with a CREB-specific antibody. Xueying Jiang et al. J. Neurosci. 2008;28:1118-1130 ©2008 by Society for Neuroscience