Genome-wide mutant fitness data for three Bacteroidetes strains.

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Genome-wide mutant fitness data for three Bacteroidetes strains. Genome-wide mutant fitness data for three Bacteroidetes strains. The fitness values for each gene were calculated using transposon insertions in the first half versus the second half (13). Each gene fitness value is a log2 ratio comparing the barcode abundance of mutants within that gene before and after growth selection. Genes highlighted in blue are listed in Table S4. TIGRFAM auxotrophs are predicted amino acid biosynthesis genes (20). (A) The mutant library of Echinicola vietnamensis (Cola_ML5) grown in defined minimal medium with glucose as the sole carbon source. (B) The mutant library of Pedobacter sp. GW460-11-11-14-LB5 (Pedo557_ML3) grown in defined minimal medium with glucose as the sole carbon source. (C) The mutant library of Pontibacter actiniarum (Ponti_ML7) grown in a defined minimal medium with a mixture of 20 amino acids (see Materials and Methods). Hualan Liu et al. mSystems 2018; doi:10.1128/mSystems.00143-17