Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. Characterization of DNA-PK and PARP-1 levels and activities in the.

Slides:



Advertisements
Similar presentations
Enhanced DNA damage in IDH1 mutant glioma cells.
Advertisements

Targeting the PARP DNA repair pathway enhanced cytotoxicity induced by chemotherapy. Targeting the PARP DNA repair pathway enhanced cytotoxicity induced.
DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived PC12 cells. DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived.
Time course of phosphorylation changes at Ser-293, Ser-300, and Ser-232 in PDHE1α following kinase inhibition with DCA. A, relative quantitation over three.
Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.
Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active.
Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/-
HIPK2 decreases the stability of Notch1-IC through proteasome-dependent degradation. HIPK2 decreases the stability of Notch1-IC through proteasome-dependent.
Changes in mutation rate or protein abundance are not observed in HATs when comparing rho+ to rho0 cells. Changes in mutation rate or protein abundance.
PARP cleavage is detected in TA cells, but not in KSC
Validation of DNMT3A splice variant expression.
IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.
The sesquiterpene lactone PN up-regulates MDM2 and p53 proteins.
Streptavidin identified in the vaccine protein profiles.
Quantification of NMT knockdown.
Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis.
Representative Western blot analyses of S-phase and other proteins from PC3 cell lysates obtained 3 days after oligomer (400 nm)/Lipofectin (15 μg/ml)
Effects of knockdown of DDR1 levels on proliferation and apoptosis.
FOXO3a phosphorylation and expression.
BME treatment increases granzyme B expression in NK 3.3 cells.
Imatinib mesylate inhibits PDGF-mediated ERK and Akt activation.
NF-kB binds to the NF-κB binding site immediately adjacent to the AT-rich regulatory region. NF-kB binds to the NF-κB binding site immediately adjacent.
ALDH1A3 is mainly responsible for the ALDH activity in two human cholangiocarcinoma lines. ALDH1A3 is mainly responsible for the ALDH activity in two human.
Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.
A, top, representative Western blot for Ku70 in MCF7 cell extracts following siRNA knockdown of Ku70, compared with siRNA control. A, top, representative.
VEGF expression in neoplastic and normal prostate tissue.
A, one-step viral growth curves: RT treatment increased the virus yield in MV-CEA–treated U87 cells by up to 2 log as compared with MV-CEA infection only.
Expression of CRC stem cell markers and L1 in CRC cells.
A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety of 24 human cell lines. A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety.
PKM2 activation was suppressed by overexpression of MnSOD during early skin carcinogenesis. PKM2 activation was suppressed by overexpression of MnSOD during.
ADU-S100–mediated tumor clearance is dependent on CD4+, CD8+ T cells, and NK cells. ADU-S100–mediated tumor clearance is dependent on CD4+, CD8+ T cells,
N-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. n-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. HEC-1-A.
Expression pattern of TLR-4 and MyD88 in EOC cells.
EGF-induced HPSE nucleolar localization induces Topo I activity in 231BR3 cells. EGF-induced HPSE nucleolar localization induces Topo I activity in 231BR3.
Combined inhibition of Wee1 and PARP causes persistent radiation-induced DNA damage. Combined inhibition of Wee1 and PARP causes persistent radiation-induced.
PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.
LRH-1 controls cell proliferation in antiestrogen-sensitive and antiestrogen-resistant breast cancer. LRH-1 controls cell proliferation in antiestrogen-sensitive.
Interaction of Cdx1 and Cdx2 with the putative Cdx-binding sites of the human DSC2 promoter. Interaction of Cdx1 and Cdx2 with the putative Cdx-binding.
Localization of oxidated thiols in A375 melanoma cells.
Repair fidelity studies with increasing concentrations of antibodies against NHEJ repair proteins. Repair fidelity studies with increasing concentrations.
Frequency of misrepair studies with increasing concentrations of antibodies against NHEJ repair proteins. Frequency of misrepair studies with increasing.
SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.
Ligation efficiency is abrogated with increasing concentration of antibodies against NHEJ proteins. Ligation efficiency is abrogated with increasing concentration.
Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.
BEZ235 inhibits DNA-PK and enhances G2–M growth arrest after radiation
Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors. Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors.
Schematic workflow of FOXO3 protein separation, proteomic analysis, and O-GlcNAc-modified site identification. Schematic workflow of FOXO3 protein separation,
Characterization of monoclonal antibody 4
Γ-H2AX foci formation in ATM−/− and DNA-dependent protein kinase catalytic subunit (DNA-PKcs)−/− mouse embryo fibroblasts (MEFs) but not in ATM−/− MEFs.
Effects of UVB on AKT in SKH-1 mouse epidermis.
Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.
Western blot analysis for bFGF expression in human glioma cell lines.
Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter. Comparison of Akt1 and Akt3 for their abilities to activate the Fra-1 promoter.
AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.
SAHA blocks IR-induced increase of RAD51 protein in MM cells.
The effects of AZD1775 and olaparib on DNA damage response signaling.
Interaction of NHEJ and HR pathways in PCO cultures.
BEZ235 induces accelerated senescence after radiation (IR) in vitro.
W. chinensis extract induces apoptosis in AR-dependent prostate cancer cells. W. chinensis extract induces apoptosis in AR-dependent prostate cancer cells.
Posttranslational phosphorylation of p53 by platinum drugs in ovarian tumor cells. Posttranslational phosphorylation of p53 by platinum drugs in ovarian.
PARP1 suppresses the transcription of PD-L1 in cancer cells.
RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis in ovarian cancer cells. RA-9 induces G2–M cell-cycle arrest and caspase-mediated apoptosis.
mRNA expression of PARP1 in SCLC cell lines and solid tumors.
Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.
Effects of NMT siRNAs on MARCKS and c-Src.
Inhibition of HDACs disrupts DNMT1 association with Hsp90.
I3C reduces the level of Cdc25A protein in breast cancer cells.
Silencing E4BP4 abrogates the promoting effect of SMAD3 knockdown on IFNγ production in NK-92 cells. Silencing E4BP4 abrogates the promoting effect of.
Sp1 complementation assay.
Time course of NMT knockdown.
Presentation transcript:

Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. A, Western blot analysis of PARP-1 and DNA-PK. Blots were probed using antibodies against PARP-1 and DNA-PKcs; B, DNA-PK activity; C, PARP-1 activity. Results are the mean of three replicate samples each from three independent experiments ± SE. Stephany J. Veuger et al. Cancer Res 2003;63:6008-6015 ©2003 by American Association for Cancer Research