BEZ235 inhibits DNA-PK and enhances G2–M growth arrest after radiation

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BEZ235 inhibits DNA-PK and enhances G2–M growth arrest after radiation. BEZ235 inhibits DNA-PK and enhances G2–M growth arrest after radiation. A, representative immunofluorescent images of phospho-DNA-PKcs (Thr2609) foci at 30 minutes after radiation (×60 magnification). Green, phospho-DNA-PKcs foci; blue, DAPI staining. Right, number of phospho-DNA-PKcs foci per cell. Data are mean ± SEM from 3 independent experiments. ***, P < 0.001; **, P < 0.01. B, clonogenic survival assays of H460 and A549 cells. Data points represent the mean surviving fraction ± SEM of 3 independent experiments. C, cell-cycle profile of H460 and A549 cells stained with propidium iodide after 48 hours treatment. Right, quantitation of sub-G1 fraction. Data are mean ± SEM from 3 independent experiments. Arun Azad et al. Mol Cancer Res 2011;9:1696-1707 ©2011 by American Association for Cancer Research