Involvement of estrogen-related receptor-γ and mitochondrial content in intrauterine growth restriction and preeclampsia  Dorothée Poidatz, Ph.D., Esther Dos Santos, Ph.D., Fabien Duval, B.S., Hadia Moindjie, B.S., Valérie Serazin, M.D., Ph.D., François Vialard, M.D., Ph.D., Philippe De Mazancourt, M.D., Ph.D., Marie-Noëlle Dieudonné, Ph.D.  Fertility and Sterility  Volume 104, Issue 2, Pages 483-490 (August 2015) DOI: 10.1016/j.fertnstert.2015.05.005 Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Mitochondrial content. (A) Mitochondrial DNA content was quantified in control (n = 30), IUGR (n = 39), and IUGR+PE (n = 8) placentas, as described in Materials and Methods. Data are reported as the mean ± SEM. *P<.05, ***P<.001. (B) Relationship between placental mitochondrial DNA content and fetal weight in the pooled control, IUGR, and IUGR+PE groups (y intercept = −20.03, slope = 40.97, P<.001). (C) Quantification of COX6c protein immunostaining on sections from control (a), IUGR (b), and IUGR+PE (c) placentas, as described in Materials and Methods. The negative control was processed in the absence of primary antibody on human control placentas (d). The figure shows one representative from five separate experiments for each group. Magnification, ×20. (e) Relative quantification of COX6c immunostaining (n = 5). Data are reported as the mean ± SEM. *P<.05, **P<.01. ns = nonsignificant. Fertility and Sterility 2015 104, 483-490DOI: (10.1016/j.fertnstert.2015.05.005) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Estrogen-related receptor-γ expression and PGC-1α and SIRT1 mRNA expressions. Total RNA was extracted from control (n = 30), IUGR (n = 39), and IUGR+PE (n = 8) placentas, as described in Materials and Methods. Estrogen-related receptor-γ1 (A), PGC-1α (B), and SIRT1 (C) mRNA expression were quantified as described in Materials and Methods. Data are reported as the mean ± SEM. *P<.05, **P<.01, ***P<.001. (D) Quantification of ERRγ protein immunostaining on sections from control (a), IUGR (b), and IUGR+PE (c) placentas, as described in Materials and Methods. The negative control was processed in the absence of primary antibody on human control placentas (d). The figure shows one representative from five separate experiments for each group. Magnification, ×20. (e) Relative quantification of ERRγ immunostaining (n = 5). Data are reported as the mean ± SEM. *P<.0.05, **P<.01. ns = nonsignificant. Fertility and Sterility 2015 104, 483-490DOI: (10.1016/j.fertnstert.2015.05.005) Copyright © 2015 American Society for Reproductive Medicine Terms and Conditions