Mig1 influences the TOR and cell wall remodeling pathways.

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Mig1 influences the TOR and cell wall remodeling pathways. Mig1 influences the TOR and cell wall remodeling pathways. (A) Resistance of the mig1Δ mutant to rapamycin/sirolimus and caffeine. Spot assay of serially diluted WT strain H99 and derivative mutants on YPD plates with 10 µg ⋅ ml−1 sirolimus and 0.5 mg ⋅ ml−1 caffeine. (B) Growth inhibition of the mig1Δ mutants by agents that challenge cell wall integrity and by elevated concentrations of salts. Spot assay of serially diluted WT strain H99 and derivative mutants on YPD plates with 0.125% SDS, 1.5 M sodium chloride (NaCl), 1.5 M potassium chloride (KCl), or 100 mM lithium chloride (LiCl). The plates were incubated at 30°C or 37°C for 2 days. Mélissa Caza et al. mSphere 2016; doi:10.1128/mSphere.00080-15