by Meritxell Jodar, Edward Sendler, Sergey I. Moskovtsev, Clifford L

Slides:

Advertisements

Similar presentations

Clonal bias of CD56−CD16+ NK cell subpopulations.

Advertisements

Christopher R. Cabanski, Vincent Magrini, Malachi Griffith, Obi L

Fig. 6. Effects of CD31-NP targeting in perfused human kidneys.

Widespread Inhibition of Posttranscriptional Splicing Shapes the Cellular Transcriptome following Heat Shock Reut Shalgi, Jessica A. Hurt, Susan Lindquist,

Transcriptome changes in Cnot6l knockout oocytes and zygotes.

The transcript profiles in the three human cell lines based on RNA sequencing (RNA‐seq). The transcript profiles in the three human cell lines based on.

Joseph Rodriguez, Jerome S. Menet, Michael Rosbash Molecular Cell

G. Charles Ostermeier, Ph. D. , Robert J. Goodrich, B. S. , Michael P

Fig. 2. Pathways differentially regulated in patients with early Lyme disease and STARI. Pathways differentially regulated in patients with early Lyme.

Volume 8, Issue 6, Pages (September 2014)

Correlation of reovirus RNA/protein with proliferating tumor cells

Fig. 7 Correlation of NHP and human ISGs.

Volume 8, Issue 6, Pages (September 2014)

Fig. 5 Correlation of RNA expression and protein abundance.

Fig. 1. Distribution of TTNtv in healthy individuals and DCM patients, and TTN exon usage in the heart. Distribution of TTNtv in healthy individuals and.

Fig. 1. Generation of the ΔEx50 mouse model.

Fig. 2. Clinically actionable somatic genomic alterations in various tumor types. Clinically actionable somatic genomic alterations in various tumor types.

Fig. 5. Microarray analysis of tumors treated by dabrafenib, trametinib, or the combination of dabrafenib and trametinib with pmel-1 ACT or mock ACT. Microarray.

Fig. 3. Comparison of prediction performance.

Taxonomic representation and metabolic potential in metagenomes

Fig. 7 Bacterial dependency networks in IgA deficiency and HDs.

Fig. 2 E2F1 affects alternative splicing of E2F target genes.

Fig. 4 Deorphanization of quinine and mefloquine protein targets.

Fig. 5 E2F1 also interacts with alternatively spliced transcripts from the MECOM gene. E2F1 also interacts with alternatively spliced transcripts from.

Fig. 1 meR marks on E2F1 confer genome-wide effects.

Fig. 2. Bacterial/viral score in COCONUT-conormalized whole-blood validation data sets. Bacterial/viral score in COCONUT-conormalized whole-blood validation.

CD25 expression identifies two transcriptionally distinct subsets of very early effector cells. CD25 expression identifies two transcriptionally distinct.

Fig. 6 Metarrestin treatment reduces pre-RNA synthesis and Pol I occupancy at rDNA without changing rDNA chromatin states. Metarrestin treatment reduces.

Fig. 5. Vitamin B12 supplementation in the host altered the transcriptome of P. acnes in the skin microbiota. Vitamin B12 supplementation in the host altered.

Fig. 5 Transcriptional changes in dKO mice after HFD regimen.

CD mediates metabolism and efflux of crystal-derived cholesterol

Fig. 4 Rational therapeutic modulation of the tumor microenvironment sensitizes tumors to ICB. Rational therapeutic modulation of the tumor microenvironment.

Fig. 2 Inflammatory pathways with STAT1 as a key regulator are enriched in ICB responsive tumors in mouse models and patients. Inflammatory pathways with.

Fig. 1. Detection of circulating tumor DNA in CRPC patients.

Fig. 3 Identification of KEECs that increase KCC2 expression in human RTT neurons. Identification of KEECs that increase KCC2 expression in human RTT neurons.

Fig. 1 Genes up-regulated in Zic5 KO cells correlate with the increased glycolytic state. Genes up-regulated in Zic5 KO cells correlate with the increased.

The microchip-based drug delivery device and overview of study design

Fig. 1. A model to explain the pleiotropy of CEP290 disease.

Fig. 1 A time-course RNA-seq analysis reveals prominent IRF8 expression in the spinal cord during the recovery phase after SCI. A time-course RNA-seq analysis.

Fig. 3 Transcriptional changes in dKO mice.

Fig. 2 PK dosing results. PK dosing results. (A) Plasma concentration of hPTH(1–34) versus time after release of 40-μg dose from implanted microchip device.

Fig. 3 Local Maraba treatment of TNBC tumors provides long-term systemic protection. Local Maraba treatment of TNBC tumors provides long-term systemic.

Fig. 1 Structure and absorption spectra of the P

by Dezhi Kang, Baochen Shi, Marie C. Erfe, Noah Craft, and Huiying Li

Representative CT and PET/CT images of three patients with NSCLCs

MHC proteins confer differential sensitivity to CTLA-4 and PD-1 blockade in untreated metastatic melanoma by Scott J. Rodig, Daniel Gusenleitner, Donald.

Fig. 2. An automated cell phone–based video microscope.

Epigenetic therapy overcomes treatment resistance in T cell prolymphocytic leukemia by Zainul S. Hasanali, Bikramajit Singh Saroya, August Stuart, Sara.

Depicting brighter possibilities for treating blindness

Fig. 5. Vascularization of human liver seed grafts.

by Christopher Ness, Romain Mari, and Michael E. Cates

Evaluation of clinical responses after infusion of CART19 cells

Delineating cancer evolution with single-cell sequencing

Fig. 2 Dynamics of Nef-stop repair and predictors of viral set-point.

Expression data from genes involved in regulation of transit through the cell cycle in response to treatment with E2 and OHT. A. Expression data from genes.

Fig. 1 Relevance of therapies for treating retinal degeneration and stage of disease. Relevance of therapies for treating retinal degeneration and stage.

Fig. 3 Characterization of SGN responses to optogenetic stimulation.

Identification of novel polymorphisms in the nuclear protein genes and their relationship with human sperm protamine deficiency and severe male infertility

Fig. 3 Effects of ASO and eGLP1-ASO conjugates on gene expression and protein levels in vitro in cell lines and primary mouse islet cells. Effects of ASO.

Fig. 3. Association between peak CTL019 expansion and response.

Fig. 1. APP/PS1;C3 KO mice show improved cognitive flexibility (reversal) compared to APP/PS1 mice at 16 months of age. APP/PS1;C3 KO mice show improved.

Fig. 1 B2 RNA directly inhibits transcription.

Fig. 2 Spatial distribution of five city groups.

Fig. 8 Immune correlates of protection.

Fig. 3 Gene expression analysis in 48-plex drug treatment experiments.

Fig. 3 Postnatal assembly of the humanized gut microbiota.

Fig. 1 Anti-LtxA antibody concentrations in various patient groups.

Fig. 2 DFT calculations of stability and bandstructure of gallenene polymorphs. DFT calculations of stability and bandstructure of gallenene polymorphs.

Cxxc1-deficient TH17 cells exhibit a Treg cell–like expression profile

Fig. 6. Connectivity and mobility maps for Lesotho.

Presentation transcript:

Absence of sperm RNA elements correlates with idiopathic male infertility by Meritxell Jodar, Edward Sendler, Sergey I. Moskovtsev, Clifford L. Librach, Robert Goodrich, Sonja Swanson, Russ Hauser, Michael P. Diamond, and Stephen A. Krawetz Sci Transl Med Volume 7(295):295re6-295re6 July 8, 2015 Copyright © 2015, American Association for the Advancement of Science

Fig. 1. Genomic localization (exon, intron, intergenic, and noncoding RNAs) and function of the 648 required SREs. Most of the required SREs are located in exons of annotated genes (585 of 648; 90.3%), and the remainder are in intronic regions (12 of 648; 1.9%), intergenic regions (9 of 648; 1.4%), or mat Genomic localization (exon, intron, intergenic, and noncoding RNAs) and function of the 648 required SREs. Most of the required SREs are located in exons of annotated genes (585 of 648; 90.3%), and the remainder are in intronic regions (12 of 648; 1.9%), intergenic regions (9 of 648; 1.4%), or match to noncoding transcriptional elements including small nuclear RNAs, microRNAs, and long noncoding RNAs (42 of 648; 6.5%) with potential regulatory function. About 40% of the genes that contain one or more SREs have a known role in spermatogenesis, sperm physiology (sperm energy production or acrosome reaction), fertilization, and/or early embryogenesis. Additionally, 20% have a known role in cellular process such as transcription regulation, protein transport, ubiquitin-like conjugation pathway, and lipid metabolism. The potential function of the remaining transcripts has yet to be defined. Meritxell Jodar et al., Sci Transl Med 2015;7:295re6 Copyright © 2015, American Association for the Advancement of Science

Fig. 2. Distribution of the 648 required SREs Fig. 2. Distribution of the 648 required SREs. The percentile rank distribution of the SREs in control (group I) and test set [group II: (i) exclusively use TIC or IUI, (ii) unsuccessful IUI followed by ART, and (iii) directly use ART] is presented. Distribution of the 648 required SREs. The percentile rank distribution of the SREs in control (group I) and test set [group II: (i) exclusively use TIC or IUI, (ii) unsuccessful IUI followed by ART, and (iii) directly use ART] is presented. The percentile rank of each element is indicated in a gradient color scale. Red indicates the complete absence of an element (zero percentile rank). In contrast, elements that are present range from yellow for the 60th abundance percentile to green that corresponds to >90th percentile of abundance. (A) (Left) Seven TIC individuals who were used to identify the SREs because they were considered to have successful natural conception and presented with all 648 SREs. (Middle) Thirty-seven samples from group II (25 group II-i, 5 group II-ii, and 7 group II-iii) with the complete set of SREs. (Right) Nineteen samples from group II (4 group II-i, 8 group II-ii, and 7 group II-iii) with at least one missing SRE. The first 33 sperm elements were absent in at least one sample from the test set (right). The remaining SREs (34 to 648) were present in all samples from groups I and II, showing that the vast majority of SREs are uniformly abundant in all samples surveyed. (B) Couples with all SREs using TIC/IUI or ART have a success rate of 72% (16 of 22) during the first two treatment cycles (6 months) after sperm RNA evaluation. Meritxell Jodar et al., Sci Transl Med 2015;7:295re6 Copyright © 2015, American Association for the Advancement of Science

Fig. 3. Analysis of treatment outcome as a function of required SREs Fig. 3. Analysis of treatment outcome as a function of required SREs. (A). Analysis of treatment outcome as a function of required SREs. (A). Most of the 37 group II couples with all SREs present underwent TIC/IUI, achieving an LB rate of 73% (22 of 30). The remaining samples reflecting patient preference along with the previously unsuccessful TIC/IUI cases were treated by ART, achieving a 75% (9 of 12) LB rate. (B) RNA analysis from samples with at least one SRE absent. Note that only 3 of the 11 TIC/IUI samples with an absent SRE achieved LB. The success ratio of LB using ART is similar to the ratio observed in samples with all SREs. (C) The percentage of LB using a noninvasive treatment for couples presenting with the complete set of SREs is higher compared to those with at least one SRE absent (two-tailed Fisher’s exact test, P = 0.012). Meritxell Jodar et al., Sci Transl Med 2015;7:295re6 Copyright © 2015, American Association for the Advancement of Science