A B Supplement Fig. 1. MMP-2 inhibition does not induce autophagy and suppress α-MSH -induced melanogenesis (A) Melan-a cells transiently transfected with GPF-LC3 were treated with various MMP-2 inhibitors (10 uM ARP101, 20 uM Actinonin, 20 uM Z-PLG-NHOH) for 24 hr, then, the autophagy was monitored. (B) Melan-a cells pre-treated with α-MSH were incubated with indicated MMP-2 inhibitors (10 uM ARP101, 20 uM Actinonin, 20 uM Z-PLG-NHOH) for 24 hr. Then, the melanin contents were measured as described in Material and Method.

A B C Supplement Fig. 2. Inhibition of autophagy increases melanin contents. (A and B) Melan-a and B16F1 cells were transfected with scrambled control siRNA (Scram) or ATG5 siRNA (siATG5), ATG7 siRNA (siATG7), and melanin contents were measured after 3 days. (C) Melan-a cells were treated with α-MSH, Bafilomycin A1 (10 nM, Baf A1), or hydroxychloroquine (50 uM, HCQ). After harvesting, the cells, cellular melanin contents were measured.