Precursor B Cells Showing H Chain Allelic Inclusion Display Allelic Exclusion at the Level of Pre-B Cell Receptor Surface Expression  Edwin ten Boekel, Fritz Melchers, Antonius G Rolink  Immunity  Volume 8, Issue 2, Pages 199-207 (February 1998) DOI: 10.1016/S1074-7613(00)80472-0

Figure 1 FACS Profiles of 38B9 Cells Transfected with μ Chains Encoded by Both Productively Rearranged Alleles from Three VDJ+/VDJ+ PreB-II Cells (A) Detection of cell surface expression of the transfected μ chains (FL1). Cell numbering is the same as in Table 3. The transfected cells were also stained in the cytoplasm and on the cell surface with a κL chain–specific mAb for the possible expression of 38B9 cell-endogenous expression of κL chains. All transfectants were found to be negative in both assays (data not shown). (B) Cytoplasmic expression of the transfected μ chains (FL1). Immunity 1998 8, 199-207DOI: (10.1016/S1074-7613(00)80472-0)

Figure 2 Early Precursor B Cell Compartments in the Bone Marrow of Normal and λ5-Deficient Mice c-Kit+CD43+CD25− preB-I cells undergoing a productive VHDJH rearrangement enter the pool of c-Kit+cμ+ cells. Pairing of this μH chain with the SL chain leads to the formation of a surface-expressed preBcR, resulting in down-regulation of c-Kit and up-regulation of CD25. Hence, these cells enter the pool of large preB-II cells. Immunity 1998 8, 199-207DOI: (10.1016/S1074-7613(00)80472-0)