Deletion of Tgfbr2 in myeloid cells elevated IFN-γ production in CD8+ T cells, and systemic IFN-γ neutralization diminished metastasis inhibition in Tgfbr2MyeKO.

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Deletion of Tgfbr2 in myeloid cells elevated IFN-γ production in CD8+ T cells, and systemic IFN-γ neutralization diminished metastasis inhibition in Tgfbr2MyeKO mice. Deletion of Tgfbr2 in myeloid cells elevated IFN-γ production in CD8+ T cells, and systemic IFN-γ neutralization diminished metastasis inhibition in Tgfbr2MyeKO mice. A, flow cytometry of IFN-γ intracellular staining of CD8+ T cells from the spleens of Tgfbr2MyeKO and Tgfbr2flox/flox mice bearing 4T1 tumors (left). Quantitative data are on the right. Shown is one of two experiments carried out. *, P < 0.05. B, IFN-γ ELISPOT of T cells from OT1 transgenic mice when cocultured with Gr-1+ myeloid cells sorted from the speens of Tgfbr2MyeKO mice bearing 4T1 tumors. Quantitative data are at the bottom. Two experiments were carried out, with each sample in triplicate. **, P < 0.01. C, lung metastasis (mets) of 4T1 tumor-bearing Tgfbr2MyeKO and Tgfbr2flox/flox mice after injection with IFN-γ neutralizing antibody or immunoglobulin G (IgG) control. *, P < 0.05; ns, not significant; Ab, antibody. Yanli Pang et al. Cancer Discovery 2013;3:936-951 ©2013 by American Association for Cancer Research