Reconstitution of the wild-type CD11b+Ly6G+ myeloid cell subset in Tgfbr2MyeKO mice reversed the diminished metastasis phenotype. Reconstitution of the.

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Reconstitution of the wild-type CD11b+Ly6G+ myeloid cell subset in Tgfbr2MyeKO mice reversed the diminished metastasis phenotype. Reconstitution of the wild-type CD11b+Ly6G+ myeloid cell subset in Tgfbr2MyeKO mice reversed the diminished metastasis phenotype. A, decreased TGF-β1 (ELISA), IL-10, and IL-4 [quantitative PCR (qPCR)] were found in CD11b+Ly6G+ cells from Tgfbr2MyeKO mice. B, qPCR showed decreased iNOS and arginase 1 in CD11b+Ly6G+ and CD11b+Ly6C myeloid cell subsets. For both A and B, myeloid subsets were sorted by fluorescence-activated cell sorting (FACS). RNA was extracted and subjected to qPCR analysis. Supernatant from cultured subsets was used for ELISA. Myeloid cells were isolated from the spleens of 4T1 tumor-bearing mice. C, NO production of myeloid cell subsets sorted from Tgfbr2MyeKO mice (C57BL/6 background), when cocultured with OVA peptide-stimulated splenocytes from OT1 mice (C57BL/6 background). For A–C, three mice each for Tgfbr2flox/flox and Tgfbr2MyeKO were examined. D, reconstitution of CD11b+Ly6G+ or Gr-1+CD11b+ cells, but not CD11b+Ly6C+ or CD11b+F4/80+ cells, diminished the decreased 4T1 tumor lung metastasis (mets) in Tgfbr2MyeKO mice (n = 7–10). All data are represented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Yanli Pang et al. Cancer Discovery 2013;3:936-951 ©2013 by American Association for Cancer Research