Depletion of Gr-1+ neutrophils by systemic anti-Gr-1 treatment prevents exacerbated neurodegeneration in RAG1-KO mice. Depletion of Gr-1+ neutrophils by systemic anti-Gr-1 treatment prevents exacerbated neurodegeneration in RAG1-KO mice. A, A′, Representative example of a control RAG1-KO mouse, administered with control IgGs. A depicts infiltrating Gr1+ neutrophils into the KA-lesioned hippocampus and A′ depicts the corresponding CD68 staining. B, B′, Example of a RAG1-KO mouse treated with anti-Gr-1 antibodies. Nissl staining (B) and CD68 immunoreactivity (B′) illustrate a lesion similar to those seen in B6 mice (Fig. 2). C, Densitometric analysis of CD68 staining at 10 d after KA in RAG1-KO mice treated with anti-Gr-1 antibodies compared with control, untreated mice (dashed line). D, Densitometric analysis of CD68 staining in KA-injected RAG1-KO mice treated with anti-Gr-1 antibodies compared with KA-injected B6 mice (dashed lines). In both cases, no single region of interest had significantly different intensity of CD68 immunoreactivity in the two groups. Error bars indicate SEM. Scale bar, 250 μm. Michela Zattoni et al. J. Neurosci. 2011;31:4037-4050 ©2011 by Society for Neuroscience