Analysis of serum TIMP3, GPX3, and TIMP1.

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Analysis of serum TIMP3, GPX3, and TIMP1. Analysis of serum TIMP3, GPX3, and TIMP1. A, total serum protein (5 μL) from normal healthy controls and GBM patients was subjected to Western blot analysis for TIMP3. B, albumin/IgG depleted serum protein (50 μg) from normal healthy controls and GBM patients was subjected to Western blot analysis for GPX3. C, serum levels of TIMP1 (ng/mL) in normal controls (n = 34), DAs (n = 13), AAs (n = 13), and GBMs (n = 52) are plotted. Horizontal line in each group designates the mean value. Statistical significance was tested by Kruskal-Wallis one-way ANOVA using SPSS 15.0 for Windows software. Peddagangannagari Sreekanthreddy et al. Cancer Epidemiol Biomarkers Prev 2010;19:1409-1422 ©2010 by American Association for Cancer Research