6-TG dose and time relationships for DSB formation using PFGE, demonstrating that the formation of DSBs in both MMR+ M4 and MMR− V2 cells is dose dependent.

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6-TG dose and time relationships for DSB formation using PFGE, demonstrating that the formation of DSBs in both MMR+ M4 and MMR− V2 cells is dose dependent but not MMR dependent. 6-TG dose and time relationships for DSB formation using PFGE, demonstrating that the formation of DSBs in both MMR+ M4 and MMR− V2 cells is dose dependent but not MMR dependent. The fragment sizes measured were approximately 1–9 Mb. A, ethidium bromide-stained gel image showing the extent of DSB formation assayed immediately after increasing doses of 6-TG for 24 h. B, ethidium bromide-stained gel image showing the time course of DSB formation after cells were treated with 3 μm 6-TG for 24 h and then harvested on day 0, 1, and 2 after 6-TG removal. C, mean fluorescence density of DNA fragments (subtracting the controls) within the same defined area analyzed with ImageJ software in B. D, ethidium bromide-stained gel image of DSB formation after treatment with 3 μm 6-TG for 24 h or immediately after 6 Gy of IR in MMR+ M4 cells. All data are representative of experiments repeated at least twice. Tao Yan et al. Clin Cancer Res 2003;9:2327-2334 ©2003 by American Association for Cancer Research